Al-Otibi Fatimah, Al-Sahli Sarah A, Alharbi Raedah I
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia.
Sci Rep. 2024 Dec 28;14(1):30987. doi: 10.1038/s41598-024-82015-4.
Rhazya stricta, a perennial shrub native to the Middle East and South Asia, has been used in traditional medicine for various therapeutic purposes, including antimicrobial action. The current study aimed to compare the antifungal properties of 96% and 50% ethanolic extracts of R. stricta leaves and their biogenic silver nanoparticles (AgNPs). The phytochemical constituents of both extracts were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The AgNPs were characterized using FTIR, UV-Vis spectroscopy, DLS, and TEM, confirming bioactive chemicals' effective production and existence. The antifungal efficacy was tested on five pathogenic fungi: Drechslera halodes, Drechslera tetramera, Macrophomina phaseolina, Alternaria alternata, and Curvularia australiensis. The 96% ethanol extract inhibited fungal growth the most, with D. halodes, C. australiensis, and A. alternata all experiencing almost 100% inhibition. AgNPs also showed strong antifungal efficacy, with those generated from 96% ethanol extract outperforming those from 50% ethanol extract. This study highlights the potential of R. stricta extracts and their biogenic AgNPs as environmentally friendly antifungal agents, which supports their use in long-term antimicrobial therapy.
刺山柑是一种原产于中东和南亚的多年生灌木，在传统医学中被用于各种治疗目的，包括抗菌作用。本研究旨在比较刺山柑叶96%和50%乙醇提取物及其生物合成银纳米颗粒（AgNPs）的抗真菌特性。通过气相色谱-质谱联用（GC-MS）分析了两种提取物的植物化学成分。使用傅里叶变换红外光谱（FTIR）、紫外-可见光谱（UV-Vis）、动态光散射（DLS）和透射电子显微镜（TEM）对AgNPs进行了表征，证实了生物活性化学物质的有效产生和存在。对五种致病真菌进行了抗真菌功效测试：盐生德氏霉、四孢德氏霉、菜豆壳球孢、链格孢和澳大利亚弯孢霉。96%乙醇提取物对真菌生长的抑制作用最强，盐生德氏霉、澳大利亚弯孢霉和链格孢的生长抑制率均接近100%。AgNPs也显示出很强的抗真菌功效，96%乙醇提取物产生的AgNPs比50%乙醇提取物产生的AgNPs表现更优。本研究突出了刺山柑提取物及其生物合成AgNPs作为环境友好型抗真菌剂的潜力，支持它们在长期抗菌治疗中的应用。
