Gupta P K, Myers J D, Baylin S B, Mulshine J L, Cuttitta F, Gazdar A F
Diagn Cytopathol. 1985 Apr-Jun;1(2):133-6. doi: 10.1002/dc.2840010209.
A new amplification technique is described for the detection of small amounts of antigen in ethanol-fixed cytologic specimens and formalin-fixed tissues. By adding a third antibody layer to the already sensitive avidin-biotin immunoperoxidase system, we have demonstrated a considerable increase in both the quantitative and qualitative aspects of this immunostaining method. In evaluating this technique, we employed a previously defined small cell lung carcinoma cell surface antigen system and monoclonal antibodies.
本文描述了一种新的扩增技术，用于检测乙醇固定的细胞学标本和福尔马林固定组织中的少量抗原。通过在已经灵敏的抗生物素蛋白-生物素免疫过氧化物酶系统中添加第三抗体层，我们证明了这种免疫染色方法在定量和定性方面都有显著提高。在评估该技术时，我们采用了先前定义的小细胞肺癌细胞表面抗原系统和单克隆抗体。
