佛波酯对来自一种分泌胰高血糖素的克隆细胞系的胰高血糖素分泌的影响。A23187与茶碱的协同作用。

Effect of phorbol esters on glucagon secretion from a glucagon-secreting clonal cell line. Synergistic effects of A23187 and theophylline.

作者信息

Ono J, Kumae S, Sato Y, Takaki R

出版信息

Diabetes Res Clin Pract. 1986 Apr;2(1):29-34. doi: 10.1016/s0168-8227(86)80026-2.

Abstract

The cell line In-R1-G9 is one of the clones from the hamster insulinoma cell line, In-111-R1, and it produces glucagon. Phorbol esters markedly enhanced glucagon secretion and the stimulatory effect was found to be correlated to their biological activity as tumor promoters. At a concentration of 200 nM, 12-O-tetradecanoylphorbol 13-acetate (TPA) stimulated glucagon secretion 13-fold more than the control in 10 min. The effect of TPA was not influenced by actinomycin D, cycloheximide, colchicine or vincristine. Depletion of calcium from the incubation medium inhibited TPA-induced glucagon secretion by approximately 50% and dibucaine also suppressed glucagon secretion to 67.4%. An addition of A23187 to TPA induced 150% enhancement over the TPA-stimulated glucagon level, and the maximum secretory response was observed when the cells were stimulated with the simultaneous addition of TPA, A23187 and theophylline.

摘要

细胞系In-R1-G9是仓鼠胰岛素瘤细胞系In-111-R1的克隆之一，它能产生胰高血糖素。佛波酯显著增强胰高血糖素的分泌，且发现这种刺激作用与其作为肿瘤促进剂的生物活性相关。在浓度为200 nM时，12-O-十四烷酰佛波醇13-乙酸酯（TPA）在10分钟内刺激胰高血糖素分泌的量比对照组多13倍。TPA的作用不受放线菌素D、环己酰亚胺、秋水仙碱或长春新碱的影响。从孵育培养基中耗尽钙可使TPA诱导的胰高血糖素分泌减少约50%，丁卡因也可将胰高血糖素分泌抑制至67.4%。向TPA中添加A23187可使胰高血糖素水平比TPA刺激时提高150%，当同时添加TPA、A23187和茶碱刺激细胞时，观察到最大分泌反应。