Ono J, Yamaguchi K, Okeda T, Asano T, Takaki R
First Department of Medicine, School of Medicine, Fukuoka University, Japan.
Diabetes Res Clin Pract. 1988 Feb 19;4(3):203-7. doi: 10.1016/s0168-8227(88)80019-6.
The In-R1-G9 cell line is one of the clones derived from the In-111-R1 hamster insulinoma cell line and produces glucagon. The secretory responses of In-R1-G9 cells were further examined to characterize the nature of the cells. Vincristine had no effect on glucagon secretion and colchicine enhanced glucagon secretion slightly after a short incubation. Two calmodulin inhibitors, trifluoperazine and chlorpromazine, did not affect glucagon secretion. Monensin at 10(-8) M suppressed glucagon secretion by 50%. Secretion of glucagon was calcium-dependent. The addition of A23187 to the incubation medium resulted in a 180% increase over control for 1 h and calcium deprivation from the medium suppressed glucagon secretion markedly. Theophylline, a phosphodiesterase inhibitor, caused a 230% increase in glucagon secretion. An experiment using cycloheximide suggested that newly synthesized glucagon appears in the medium at 30 min. This cell line should be useful for various experiments in many fields of research.
In-R1-G9细胞系是从In-111-R1仓鼠胰岛素瘤细胞系衍生而来的克隆之一，可产生胰高血糖素。对In-R1-G9细胞的分泌反应进行了进一步研究，以表征细胞的性质。长春新碱对胰高血糖素分泌无影响，秋水仙碱在短暂孵育后可轻微增强胰高血糖素分泌。两种钙调蛋白抑制剂，即三氟拉嗪和氯丙嗪，不影响胰高血糖素分泌。10(-8) M的莫能菌素可使胰高血糖素分泌抑制50%。胰高血糖素的分泌是钙依赖性的。向孵育培养基中添加A23187导致1小时内比对照增加180%，而培养基中钙的缺乏则显著抑制胰高血糖素分泌。磷酸二酯酶抑制剂茶碱可使胰高血糖素分泌增加230%。一项使用环己酰亚胺的实验表明，新合成的胰高血糖素在30分钟时出现在培养基中。该细胞系应可用于许多研究领域的各种实验。
