Kwiatos Natalia, Jędrzejczak-Krzepkowska Marzena, Strzelecki Bartosz, Bielecki Stanisław
Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 4/10, 90-924, Lodz, Poland.
AMB Express. 2018 Aug 22;8(1):133. doi: 10.1186/s13568-018-0669-1.
Clean coal technologies (e.g. coal biosolubilization) are of essential value, especially in Europe, where coal is the national wealth and other energy sources like crude oil are not available. Fusarium oxysporum LOCK 1134, the strain isolated from brown coal, efficiently biosolubilizes lignite. The obtained liquefied products contain 50% less sulfur and over 99% less mercury than the crude coal. Moreover, the liquefied coal can be modified further by laccase. In this study F. oxysporum laccase was expressed in Pichia pastoris for the first time and was assessed as an additional agent for coal degradation. The novel laccase contributes to humic and fulvic acids release from liquefied coal due to introduction of oxygen into coal structure. The effect is increased when a natural redox mediator, sinapic acid, is present in the reaction mixture-up to 30% and 80% respectively. Humic acids obtained by biological process are environmentally friendly fertilizers that may have stimulating effects on crop growth.
清洁煤技术（如煤炭生物溶解）具有重要价值，尤其是在欧洲，煤炭是国家财富，而原油等其他能源无法获取。从褐煤中分离出的尖孢镰刀菌LOCK 1134能够高效地生物溶解褐煤。所得到的液化产物相比原煤含硫量减少50%，汞含量减少99%以上。此外，液化煤还可以通过漆酶进一步改性。在本研究中，尖孢镰刀菌漆酶首次在毕赤酵母中表达，并被评估为煤炭降解的一种辅助剂。由于向煤炭结构中引入了氧，这种新型漆酶有助于从液化煤中释放腐殖酸和富里酸。当反应混合物中存在天然氧化还原介质芥子酸时，这种效果会增强，腐殖酸和富里酸的释放量分别增加至30%和80%。通过生物过程获得的腐殖酸是对环境友好的肥料，可能对作物生长有促进作用。
