Philp N J, Grollman E F
FEBS Lett. 1986 Jul 7;202(2):193-6. doi: 10.1016/0014-5793(86)80685-8.
Hormone-induced changes in phospholipid metabolism were examined in a functioning rat thyroid cell line (FRTL-5). Stimulation of FRTL-5 cells, prelabeled with 32P, with TSH or NE resulted in a rapid decrease in the radioactivity of both phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 4-monophosphate (PIP). The effects of TSH on phospholipid metabolism and calcium mobilization are independent of those on adenylate cyclase. This suggests that the TSH receptor may be unique in that it activates enzyme cascades involved in cAMP production and Ca2+ mobilization.
在一个具有功能的大鼠甲状腺细胞系（FRTL-5）中检测了激素诱导的磷脂代谢变化。用促甲状腺激素（TSH）或去甲肾上腺素（NE）刺激预先用32P标记的FRTL-5细胞，导致磷脂酰肌醇4,5-二磷酸（PIP2）和磷脂酰肌醇4-单磷酸（PIP）的放射性迅速降低。TSH对磷脂代谢和钙动员的作用独立于其对腺苷酸环化酶的作用。这表明TSH受体可能是独特的，因为它激活参与环磷酸腺苷（cAMP）产生和钙离子（Ca2+）动员的酶级联反应。
