在严重肥胖个体中关键代谢生物标志物 PGC-1α 的组织间表达模式：在肥胖相关疾病中的意义。
Inter-tissue expression patterns of the key metabolic biomarker PGC-1α in severely obese individuals: Implication in obesity-induced disease.
机构信息
Department of Pathology, Medical School, University of Patras, 26500 Patras, Greece; Second Department of Internal Medicine, Research Unit and Diabetes Center, Attikon University Hospital, National and Kapodistrian University of Athens, Medical School, Rimini 1, Haidari, 12462 Athens, Greece.
Department of Pathology, Medical School, University of Patras, 26500 Patras, Greece.
出版信息
Hellenic J Cardiol. 2019 Sep-Oct;60(5):282-293. doi: 10.1016/j.hjc.2018.08.002. Epub 2018 Aug 20.
OBJECTIVE
PGC-1α is already known as a significant regulator of mitochondrial biogenesis, oxidative phosphorylation and fatty acid metabolism. Our study focuses on the role of PGC1α in morbid obesity, in five different tissues, collected from 50 severely obese patients during planned bariatric surgery.
METHODS
The investigated tissues included subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), skeletal muscle (SM), extramyocellular adipose tissue (EMAT) and liver. PGC1α expression was investigated with immunohistochemistry and evaluated with microscopy.
RESULTS
Our findings highlighted significant positive inter-tissue correlations regarding PGC-1α expression between several tissue pairs (VAT-SAT, VAT-SM, VAT-EMAT, SAT-SM, SAT-EMAT, SM-EMAT). Moreover, we found significant negative correlations between PGC1α expression in VAT with CD68 expression in skeletal muscle and EMAT, implying a possible protective role of PGC1α against obesity-induced inflammation.
CONCLUSION
Unmasking the inter-tissue communication networks regarding PGC-1α expression in morbid obesity, will give more insight into its significant role in obesity-induced diseases. PGC1α could potentially represent a future preventive and therapeutic target against obesity-induced disease, probably through enhancing mitochondrial biogenesis and metabolism.
目的
PGC-1α 已被证实为调节线粒体生物发生、氧化磷酸化和脂肪酸代谢的关键因子。我们的研究集中于 PGC1α 在病态肥胖中的作用，在计划行减重手术时，从 50 名严重肥胖患者的 5 种不同组织中进行研究。
方法
研究的组织包括皮下脂肪组织（SAT）、内脏脂肪组织（VAT）、骨骼肌（SM）、细胞外脂肪组织（EMAT）和肝脏。通过免疫组织化学法检测 PGC1α 的表达，并通过显微镜进行评估。
结果
我们的发现强调了 PGC-1α 在几种组织对之间表达的显著正组织间相关性（VAT-SAT、VAT-SM、VAT-EMAT、SAT-SM、SAT-EMAT、SM-EMAT）。此外，我们发现 VAT 中的 PGC1α 表达与骨骼肌和 EMAT 中 CD68 的表达呈显著负相关，这意味着 PGC1α 可能对肥胖诱导的炎症具有保护作用。
结论
揭示病态肥胖中 PGC-1α 表达的组织间通讯网络，将深入了解其在肥胖相关疾病中的重要作用。PGC1α 可能通过增强线粒体生物发生和代谢，成为肥胖相关疾病的未来预防和治疗靶点。
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