Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Research Institute of Sciences and New Technology, Mashhad, Iran.
Biosens Bioelectron. 2018 Nov 15;119:204-208. doi: 10.1016/j.bios.2018.08.024. Epub 2018 Aug 11.
In this study, a novel electrochemical sensing strategy was developed based on nontarget-induced bridge assembly and aptamer (Apt) extension reaction triggered by terminal deoxynucleotidyl transferase (TdT). Bisphenol A (BPA) was chosen as the target analyte to study the analytical performance of the designed aptasensor. This sensing system takes advantages of electrochemical sensing platforms and detection of an ultra-low level of BPA, due to the formation of bridge on the surface of electrode in the absence of BPA. In the presence of BPA, Apt/BPA complex is formed and inhibits the access of TdT to the 3'-end of Apt, resulting in the lack of bridge assembly and high access of [Fe(CN)] as redox agent to the electrode surface. Thus, a strong redox peak is observed. This aptasensor enabled the selective detection of BPA with the wide linear range of 0.08-15 nM and detection limit as low as 15 pM. Furthermore, the presented sensing platform was successfully applied for the detection of BPA in tap water and grape juice samples.
在这项研究中，我们开发了一种基于非目标诱导桥联和末端脱氧核苷酸转移酶（TdT）引发的适体（Apt）延伸反应的新型电化学传感策略。双酚 A（BPA）被选为目标分析物，以研究设计的适体传感器的分析性能。由于在不存在 BPA 的情况下，电极表面形成桥，因此该传感系统利用电化学传感平台和对超痕量 BPA 的检测来实现。在存在 BPA 的情况下，Apt/BPA 复合物形成并抑制 TdT 进入 Apt 的 3'-末端，导致桥联组装的缺乏和[Fe(CN)]作为氧化还原试剂高进入电极表面。因此，观察到强的氧化还原峰。该适体传感器能够选择性地检测 BPA，线性范围为 0.08-15 nM，检测限低至 15 pM。此外，所提出的传感平台成功地应用于自来水中和葡萄汁样品中 BPA 的检测。
