Key Laboratory of Animal Resistance Biology of Shandong Province, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan 250014, PR China.
Key Laboratory of Animal Resistance Biology of Shandong Province, Institute of Biomedical Sciences, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan 250014, PR China.
Food Chem. 2020 Jul 1;317:126459. doi: 10.1016/j.foodchem.2020.126459. Epub 2020 Feb 20.
The widespread exposure of bisphenol A (BPA) presents a significant risk to human health. A rapid, ultra-sensitive and label-free colorimetric aptasensor using high affinity truncated aptamers was developed for BPA detection. Truncated 38-mer and 12-mer aptamers specific for BPA were obtained through rationally truncation from 63-mer BPA aptamer. The dissociation constants (K) of 38-mer and 12-mer aptamers were determined to be 13.17 nM and 27.05 nM. Then, truncated aptamers were used in label-free colorimetric detection assays based on gold nanoparticles (AuNPs). The limit of detections of aptasensors using 38-mer and 12-mer aptamers were 7.60 pM and 14.41 pM, which were 265-fold and 140-fold lower than that of the aptasensor using 63-mer aptamer, respectively. The recovery rates in milk, orange juice and mineralized water samples were 93.88% to 107.30%. Therefore, the developed BPA colorimetric aptasensor using truncated aptamers has great application prospects in food safety control and environmental monitoring.
双酚 A（BPA）的广泛暴露对人类健康构成重大风险。本研究开发了一种使用高亲和力截断适体的快速、超灵敏和无标记比色适体传感器，用于 BPA 的检测。通过从 63 -mer BPA 适体合理截断获得了针对 BPA 的特异性 38-mer 和 12- mer 截断适体。38- mer 和 12- mer 适体的解离常数（K）分别确定为 13.17 nM 和 27.05 nM。然后，基于金纳米粒子（AuNPs），将截断适体用于无标记比色检测分析。使用 38- mer 和 12- mer 适体的适体传感器的检测限分别为 7.60 pM 和 14.41 pM，分别比使用 63- mer 适体的适体传感器低 265 倍和 140 倍。在牛奶、橙汁和矿泉水样品中的回收率为 93.88%至 107.30%。因此，使用截断适体开发的 BPA 比色适体传感器在食品安全控制和环境监测方面具有广阔的应用前景。
