Chemical and Materials Engineering Department, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Laboratory of Cellulose and Paper, INIA, Forest Research Center, 28040 Madrid, Spain.
Bioresour Technol. 2018 Nov;268:592-598. doi: 10.1016/j.biortech.2018.08.045. Epub 2018 Aug 16.
A physico-chemical kinetic model for the hydrolysis of pre-treated corn stover is proposed. This model takes into account two reactions in series, the hydrolysis of cellulose to cellobiose and the production of glucose from cellobiose. Experiments have been carried out with an industrial enzymatic cocktail from Trichoderma reesei containing endo and exoglucanases and a very low activity of β-glucosidase. Kinetic parameters were calculated by fitting the proposed model to experimental data of cellulose and glucose concentrations with time. The kinetic parameters fulfilled all relevant statistical and physical criteria. The kinetic model has been validated with published saccharification data regarding differently pre-treated corn stover and enzymatic cocktail, in this case with a very high β-glucosidase activity (as it is common in modern industrial cellulase cocktails). In both cases, the kinetic model proposed could be fitted very appropriately to cellulose hydrolysis data.
提出了一种预处理玉米秸秆水解的物理化学动力学模型。该模型考虑了两个串联反应，即纤维素水解为纤维二糖和纤维二糖生成葡萄糖。实验采用了一种来自里氏木霉的工业酶制剂混合物，其中含有内切葡聚糖酶和外切葡聚糖酶，β-葡萄糖苷酶的活性非常低。通过将所提出的模型拟合到纤维素和葡萄糖浓度随时间变化的实验数据，计算了动力学参数。动力学参数满足所有相关的统计和物理标准。该动力学模型已经通过不同预处理玉米秸秆和酶制剂混合物的已发表糖化数据进行了验证，在这种情况下，β-葡萄糖苷酶的活性非常高（因为这在现代工业纤维素酶混合物中很常见）。在这两种情况下，所提出的动力学模型都可以很好地拟合纤维素水解数据。
