Ekert H, Chavin S I
Br J Haematol. 1977 Jun;36(2):271-9. doi: 10.1111/j.1365-2141.1977.tb00648.x.
Therapeutic factor-VIII concentrates were found to have factor VIII-related antigen (FVIIIRAg) with an electrophoretic mobility faster than that of plasma using two-dimensional crossed immunoelectrophoresis. Immediately after infusion of factor-VIII concentrates into patients, the electrophoretic mobility of FVIIIRAg in the patients' plasmas was increased to that of the antigen in the infused concentrates. Two hours after infusion, a proportion of the antigen had an electrophoretic mobility intermediate between that of the pre-infusion antigen and that of the concentrate antigen, and by 24 h after infusion the reversion of electrophoretic mobility to pre-infusion values was complete. The return of electrophoretic mobility to normal did not occur in vitro after 24 h. In vitro mixing experiments between pre-infusion plasma and concentrate resulted in antigen with a range of intermediate mobilities which were related to the relative proportions of slow and fast antigen in the mixture. In vitro mixing experiments with slow and fast antigen separated from intermediate purity factor VIII concentrate by agarose gel filtration resulted in the formation of a relatively large proportion of antigen with intermediate electrophoretic mobility. The most reasonable interpretation of the results is the formation of hybrids between the two electrophoretically different populations of antigen. This implies that VIIIRAg normally exists in a polymeric form which can spontaneously dissociate into the exist in equilibrium with a pool of partially and/or completely dissociated subunits.
采用二维交叉免疫电泳法发现，治疗性因子VIII浓缩物中的因子VIII相关抗原（FVIIIRAg）的电泳迁移率比血浆中的快。将因子VIII浓缩物注入患者体内后，患者血浆中FVIIIRAg的电泳迁移率立即升至注入浓缩物中抗原的迁移率。注入后两小时，一部分抗原的电泳迁移率介于注入前抗原和浓缩物抗原之间，注入后24小时，电泳迁移率完全恢复到注入前的值。24小时后，体外未出现电泳迁移率恢复正常的情况。注入前血浆与浓缩物的体外混合实验产生了一系列具有中间迁移率的抗原，这些迁移率与混合物中慢抗原和快抗原的相对比例有关。用琼脂糖凝胶过滤从中间纯度的因子VIII浓缩物中分离出的慢抗原和快抗原进行体外混合实验，结果形成了相对较大比例的具有中间电泳迁移率的抗原。对结果最合理的解释是两种电泳性质不同的抗原群体之间形成了杂交体。这意味着VIIIRAg通常以聚合物形式存在，该聚合物可自发解离成部分和/或完全解离的亚基池，并与之处于平衡状态。
