Hultin M B, Abildgaard U
Thromb Res. 1987 Jan 15;45(2):175-82. doi: 10.1016/0049-3848(87)90171-x.
These studies were performed to investigate the cause(s) of the cathodal shift of mobility seen in crossed immunoelectrophoresis of antithrombin antigen in plasma of hemophilic patients after factor IX concentrate therapy. These plasmas were shown to contain antithrombin neoantigen with apparent identity to the neoantigen present in normal serum but not present in normal plasma. Sephacryl S-200 gel chromatography of serum demonstrated that the neoantigen eluted with the first two, early eluting protein peaks; thus the neoantigen had a higher molecular weight than native antithrombin. When the chromatographic fractions containing the neoantigen were studied by crossed immunoelectrophoresis, they were found to contain antithrombin antigen of more cathodal mobility than normal. Sephacryl S-200 chromatography of factor IX concentrate-treated hemophilic plasma also showed an early eluting peak of antithrombin antigen of more cathodal mobility than normal in crossed immunoelectrophoresis. The mobility of this peak was identical to the cathodal peak found in normal serum and in early eluting fractions from chromatography of normal serum. These results support the conclusion that factor IX concentrate-treated hemophilic plasma contained a non-functional, high molecular weight form of antithrombin, associated with the presence of neoantigen, which may represent complexed and/or modified antithrombin produced by the action of the concentrates in vivo.
开展这些研究是为了探究在血友病患者接受凝血因子IX浓缩物治疗后，其血浆中抗凝血酶抗原交叉免疫电泳中出现的迁移率阴极偏移的原因。这些血浆显示含有抗凝血酶新抗原，与正常血清中存在但正常血浆中不存在的新抗原明显相同。血清的Sephacryl S - 200凝胶色谱显示，该新抗原与最初的两个早期洗脱蛋白峰一起洗脱；因此，该新抗原的分子量高于天然抗凝血酶。当通过交叉免疫电泳研究含有新抗原的色谱馏分时，发现它们含有迁移率比正常抗凝血酶抗原更偏向阴极的抗凝血酶抗原。对接受凝血因子IX浓缩物治疗的血友病患者血浆进行Sephacryl S - 200色谱分析，在交叉免疫电泳中也显示出一个抗凝血酶抗原的早期洗脱峰，其迁移率比正常情况更偏向阴极。该峰的迁移率与正常血清中以及正常血清色谱早期洗脱馏分中发现的阴极峰相同。这些结果支持以下结论：接受凝血因子IX浓缩物治疗的血友病患者血浆含有一种无功能的高分子量抗凝血酶形式，与新抗原的存在有关，这可能代表浓缩物在体内作用产生的复合和/或修饰的抗凝血酶。
