Abudusalamu Aini, Tuerhongjiang Tuxun, Ma Hai-zhang, Zhang Heng, Zhang Hao, Abudukaiyoumu Maimaiti, Li Yu-peng, Shadike Apaer, Lin Ren-yong, Shao Ying-mei, Wen Hao
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2016 Dec;34(6):542-6.
To investigate the expression of Toll-like receptor 2（TLR2） and TLR4 mRNA in peripheral blood mononuclear cells （PBMC） and in the liver of patients with hepatic alveolar echinococcosis (HAE), and their correlations with related cytokines in plasma.
Twenty-eight HAE patients hospitalized in the First Affiliated Hospital of Xinjiang Medical University during January 2012 and June 2015 and 28 healthy volunteers as a control were enrolled in this study. Plasma levels of interferon-γ （IFN-γ）, interleukin-5 （IL-5）, IL-23, and IL-10 were measured by ELISA. qRT-PCR was performed to detect TLR2 and TLR4 mRNA levels in PBMCs and hepatic tissues. The percentage of peripheral blood eosinophil （Eo%） was determined by a hematology analyzer. The correlations of TLR2 and TLR4 mRNA levels in PBMCs with levels of related cytokines and Eo% were analyzed with the Spearman Correlation method.
ELISA results showed that the plasma levels of IFN-γ, IL-5, IL-23, and IL-10 in the HAE group were （301.100±47.290）, （43.420±11.380）, （86.580±31.990） and （8.766±7.568） pg/ml respectively, which were higher than those in the control［（301.100±67.790）, （40.970±6.310）, （46.770±15.490） and （6.272±10.360） pg/ml］ with a statistical significance for IL-23 （P<0.01）. Results of qRT-PCR showed that the expression level of TLR2 in the HAE group （0.100±0.084） was significantly higher than that in the control （0.055±0.040） （P<0.05）, while the expression level of TLR4 in the HAE group （0.004±0.003） was comparable to that in the control（0.003±0.002）（P>0.05）. The expression of TLR2 and TLR4 mRNA in HAE lesions in the HAE group（29.680±25.650 and 21.340±16.640, respectively） were both significantly higher than that in para-lesion regions（2.308±4.140 and 5.541±9.233） and that in tissues of the control （1.112±1.431 and 1.100±1.734）（P<0.01）. There was also a significant difference in Eo% between the HAE（0.448±0.240） and the control（0.110±0.100） groups. Spearman correlation coefficients revealed a positive correlation of TLR2 mRNA in PBMCs with plasma IL-23 level and peripheral blood Eo% in HAE subjects（r=0.368, r=0.382, respectively）.
There are increases in TLR2 and TLR4 mNRA expression in PBMCs and in HAE lesions in HAE patients. The TLR2 mNRA expression in PBMCs positively correlates with plasma IL-23 level and peripheral Eo%.
探讨肝泡型包虫病（HAE）患者外周血单个核细胞（PBMC）及肝脏中Toll样受体2（TLR2）和TLR4 mRNA的表达情况，及其与血浆中相关细胞因子的相关性。
选取2012年1月至2015年6月在新疆医科大学第一附属医院住院的28例HAE患者及28名健康志愿者作为对照。采用酶联免疫吸附测定（ELISA）法检测血浆中干扰素-γ（IFN-γ）、白细胞介素-5（IL-5）、IL-23和IL-10的水平。采用实时荧光定量聚合酶链反应（qRT-PCR）检测PBMC和肝组织中TLR2和TLR4 mRNA水平。采用血液分析仪检测外周血嗜酸性粒细胞百分比（Eo%）。采用Spearman相关分析法分析PBMC中TLR2和TLR4 mRNA水平与相关细胞因子水平及Eo%的相关性。
ELISA结果显示，HAE组血浆中IFN-γ、IL-5、IL-23和IL-10水平分别为（301.100±47.290）、（43.420±11.380）、（86.580±31.990）和（8.766±7.568）pg/ml，均高于对照组［（301.100±67.790）、（40.970±6.310）、（46.770±15.490）和（6.272±10.360）pg/ml］，其中IL-23差异有统计学意义（P<0.01）。qRT-PCR结果显示，HAE组TLR2表达水平（0.100±0.084）显著高于对照组（0.055±0.040）（P<0.05），而HAE组TLR4表达水平（0.004±0.003）与对照组（0.003±0.002）相当（P>0.05）。HAE组HAE病灶中TLR2和TLR4 mRNA表达水平（分别为29.680±25.650和21.340±16.640）均显著高于病灶旁组织（2.308±4.140和5.541±9.233）及对照组组织（1.112±1.431和1.100±1.734）（P<0.01）。HAE组（0.448±0.240）与对照组（0.110±0.100）的Eo%也有显著差异。Spearman相关系数显示，HAE患者PBMC中TLR2 mRNA与血浆IL-23水平及外周血Eo%呈正相关（r分别为0.368和0.382）。
HAE患者PBMC及HAE病灶中TLR2和TLR4 mRNA表达增加。PBMC中TLR2 mRNA表达与血浆IL-23水平及外周血Eo%呈正相关。
