College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao 266071, PR China.
College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao 266071, PR China.
Biosens Bioelectron. 2018 Nov 30;120:15-21. doi: 10.1016/j.bios.2018.08.031. Epub 2018 Aug 15.
In this protocol, a dual-channel homogeneous aptasenor was proposed for protein molecule determination, employing thrombin as target analyte. The colorimetric and electrochemical transducers were combined in a single analytical system for signal readout. In this dual-channel sensing strategy, the G-quadruplex sequence was released and incorporated with hemin to form DNAzyme for naked-eye colorimetric detection. Meanwhile, the hydroxyapatite nanoparticle as signal probe was combined with magnetic nanoparticles to construct sandwich-type structure for generating the electrochemical current when thrombin was present in solution. By introducing two kinds of reporter probes and transducers, this dual-channel sensor produced two different kinds of signal to improve the analytical accuracy and diversity. The results revealed that the dual-channel sensor achieved the quantatitive determination of thrombin with low limit of detection (0.40 fM) and wide range (0.1 fM to 1 nM), which offer a promise for rapid and accurate detection of biomolecule.
本方案提出了一种双通道均相适体传感器，用于蛋白质分子测定，以凝血酶为目标分析物。比色和电化学换能器被组合在单个分析系统中用于信号读出。在这种双通道传感策略中，G-四链体序列被释放并与血红素结合形成 DNA 酶，用于肉眼比色检测。同时，羟基磷灰石纳米颗粒作为信号探针与磁性纳米颗粒结合，构建三明治结构，当溶液中有凝血酶存在时产生电化学电流。通过引入两种报告探针和换能器，双通道传感器产生了两种不同的信号，以提高分析的准确性和多样性。结果表明，双通道传感器实现了凝血酶的定量测定，具有较低的检测限（0.40 fM）和较宽的检测范围（0.1 fM 至 1 nM），为生物分子的快速准确检测提供了可能。
