College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao, 266071, People's Republic of China.
College of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao, People's Republic of China.
Mikrochim Acta. 2019 Aug 30;186(9):659. doi: 10.1007/s00604-019-3781-2.
A homogeneous electrochemical aptasensor was obtained by modifying a glassy carbon electrode (GCE) with a porous carbon nanomaterial (Z-1000, about 70 nm, deteced by transmission electron microscopic) that was obtained by carbonization of a zinc(II)-2-methylimidazole metal-organic framework. Z-1000 possesses a large specific surface and outstanding electrochemical properties. A thrombin-binding aptamer (CP) was immobilized on the magnetite nanoparticles MNPs by the condensation reaction and further combined with reporter probe (RP) that is functionalized with electroactive methylene blue (MB). In the presence of thrombin, the CP was specifically recognized with it to form the CP/MNP/Thb complex, and the RP was dissociated from MNPs. The released RP was captured by the modified GCE through π-stacking interaction between nucleobases and carbon nanostructure. The electrical signal generated by MB can be monitored by differential pulse voltammetry (DPV). Under the optimized conditions, the DPV peak current at around -0.28 V (vs. SCE) increases with thrombin concentration. The sensor has a detection limit of 0.8 fM of thrombin and a linear range that extends from 10 fM to 100 nM. It was successfully applied to the analysis of spiked serum. The recoveries are 98.1-99.4% and RSDs are 3.9%-4.0%. Conceivably, this aptasensor scheme can be easily extended to other proteins and gives inspiration to manufacture sensitive aptasensor. Graphical abstract A homogeneous electrochemical aptasensor is obtained by modifying a glassy carbon electrode with the MOF-derived porous carbon. The sensor has a detection limit of 0.8 fM and a wide linear range from 10 fM to 100 nM for thrombin detection.
一种均相电化学适体传感器是通过修饰玻碳电极（GCE）得到的，修饰方法是使用一种多孔碳纳米材料（Z-1000，约 70nm，通过透射电子显微镜检测），Z-1000 是由锌（II）-2-甲基咪唑金属有机骨架碳化得到的。Z-1000 具有大的比表面积和优异的电化学性能。凝血酶结合适体（CP）通过缩合反应固定在磁性纳米颗粒 MNPs 上，然后与功能化的电活性亚甲基蓝（MB）的报告探针（RP）结合。在凝血酶存在下，CP 特异性识别它，形成 CP/MNP/Thb 复合物，RP 从 MNPs 上解离。释放的 RP 通过碱基与碳纳米结构之间的π-堆积相互作用被修饰的 GCE 捕获。通过差分脉冲伏安法（DPV）可以监测 MB 产生的电信号。在优化条件下，约-0.28V（相对于 SCE）处的 DPV 峰电流随凝血酶浓度的增加而增加。该传感器对凝血酶的检测限为 0.8 fM，线性范围从 10 fM 扩展到 100 nM。它已成功应用于加标血清的分析。回收率为 98.1%-99.4%，相对标准偏差为 3.9%-4.0%。可以想象，这种适体传感器方案可以很容易地扩展到其他蛋白质，并为制造灵敏的适体传感器提供启示。
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