Caulford P G, Coceani F
Can J Physiol Pharmacol. 1977 Apr;55(2):293-300. doi: 10.1139/y77-042.
5-Hydroxytryptamine (5-HT) and epinephrine were applied by microiontophoresis to single neurons in the isolated spinal cord of the frog. 5-HT depressed all but two of the responsive cells, whereas the response to epinephrine consisted exclusively of depression. 5-HT action was more marked than that of epinephrine on most cells. With either compound, responseve units were diffusely distributed throughout the tissue. While it was proven that prostaglandin E1 (PGE1) exerts a direct excitatory action on spinal neurons, no evidence of an antagonism between PGE1 and the monoamines was obtained. These findings provide additional support to the hypothesis that 5-HT and epinephrine are transmitters in the frog spinal cord. The possibility that PGE1 may 'modulate' the responsiveness of spinal neurons to the monoamines was not confirmed.
通过微离子电泳法将5-羟色胺（5-HT）和肾上腺素施加于青蛙离体脊髓的单个神经元。5-HT使除两个反应性细胞外的所有细胞均受到抑制，而对肾上腺素的反应则完全是抑制作用。在大多数细胞上，5-HT的作用比肾上腺素更为显著。使用这两种化合物时，反应性单位在整个组织中呈弥散分布。虽然已证明前列腺素E1（PGE1）对脊髓神经元有直接兴奋作用，但未发现PGE1与单胺类之间存在拮抗作用的证据。这些发现为5-HT和肾上腺素是青蛙脊髓中的神经递质这一假说提供了额外支持。PGE1可能“调节”脊髓神经元对单胺类反应性的可能性未得到证实。
