Niu Chang-Chun, Wan Ya-Fang, Yang Cheng, Li Tian, Liao Pu
Department of Laboratory Medicine, Chongqing General Hospital Department of Hematology Medicine, Xinqiao Hospital, Chongqing, China.
Medicine (Baltimore). 2018 Aug;97(34):e11963. doi: 10.1097/MD.0000000000011963.
It was demonstrated in previous studies that cysteine-rich angiogenic inducer 61 (Cyr61) plays vital roles in hematological disorders, and we have already reported that the Cyr61 protein is a tumor promoter in acute myeloid leukemia (AML). Here, we investigated the association between CYR61 gene polymorphisms and susceptibility to AML.We genotyped 2 single-nucleotide polymorphisms (rs2297141 and rs6576776) in the region of the CYR61 gene by improved multiplex ligase detection reaction genotyping assays in a total of 275 samples, including samples from 137 AML patients and 138 healthy controls. Chi-squared tests and logistic regression analysis were performed to compare the different distributions of the genotypes and alleles between patients and healthy controls.The rs2297141 A allele was associated with lower risk of AML compared with the G allele (odds ratio [OR] = 0.704, 95% confidence interval [CI] = 0.503-0.985, P = .04) in both the dominant (OR = 0.447, 95% CI = 0.22-0.909, P = .025, AA vs GG) and recessive inheritance models (OR = 0.419, 95% CI = 0.23-0.763, P = .004, AA vs GA + GG). Although the distribution of the rs6576776 alleles was not different between patients with AML and normal controls, the CC genotype significantly increased the risk of AML in the dominant inheritance model (OR = 6.064, 95% CI = 1.303-28.216, P = .01, CC vs GG) and the recessive inheritance model (OR = 5.937, 95% CI = 1.291-27.306, P = .01, CC vs GC + GG). Additionally, it was shown that the rs2297141 and rs6576776 genotypes were associated with AML-M5 and AML-M2, respectively.Our findings indicated that genetic polymorphisms in the CYR61 gene may be considered potential AML risk factors in the Han Chinese population.
先前的研究表明，富含半胱氨酸的血管生成诱导因子61（Cyr61）在血液系统疾病中起重要作用，并且我们已经报道Cyr61蛋白是急性髓系白血病（AML）中的一种肿瘤促进因子。在此，我们研究了CYR61基因多态性与AML易感性之间的关联。我们通过改进的多重连接酶检测反应基因分型检测法，对总共275个样本（包括137例AML患者和138例健康对照的样本）中的CYR61基因区域的2个单核苷酸多态性（rs2297141和rs6576776）进行基因分型。进行卡方检验和逻辑回归分析，以比较患者和健康对照之间基因型和等位基因的不同分布。在显性（优势比[OR] = 0.447，95%置信区间[CI] = 0.22 - 0.909，P = 0.025，AA与GG）和隐性遗传模型（OR = 0.419，95% CI = 0.23 - 0.763，P = 0.004，AA与GA + GG）中，与G等位基因相比，rs2297141的A等位基因与较低的AML风险相关（OR = 0.704，95% CI = 0.503 - 0.985，P = 0.04）。尽管rs6576776等位基因在AML患者和正常对照之间的分布没有差异，但在显性遗传模型（OR = 6.064，95% CI = 1.303 - 28.216，P = 0.01，CC与GG）和隐性遗传模型（OR = 5.937，95% CI = 1.291 - 27.306，P = 0.01，CC与GC + GG）中，CC基因型显著增加了AML的风险。此外，结果表明rs2297141和rs6576776基因型分别与AML-M5和AML-M2相关。我们的研究结果表明，CYR61基因中的遗传多态性可能被认为是中国汉族人群中潜在的AML危险因素。
