Lomidze N V, Kramerov D A, Ryskov A P
Mol Biol (Mosk). 1986 May-Jun;20(3):761-6.
Sequences homologous to short repeated elements of the mouse genome, B1 and B2, were detected in DNA of different organisms by dot-hybridization. The sequences B1 and B2 hybridized most efficiently with DNA of Myomorpha rodents and primates. The hybridization was also observed with DNA of all other eukaryotes studied, however, it is probably caused by an existence of short homologies with the B1 and B2 sequences only. The effective hybridization of the B1 sequence with DNA of primates is apparently explained by a presence of numerous copies of Alu sequence in their genomes. A repeated sequence of the human DNA that is able to hybridize with B2 sequence was cloned. It was designated as HsB2 sequence. There are about 5000 copies of this sequence in the human genome. To estimate the degree of homology, the melting temperature of hybrids of sequences B1 and B2 with DNA of rodents and some other mammals was measured. It was found that the degree of homology of B2 sequence (but not B1) correlated well with the phylogenetic relationship of the organisms. Perhaps, the difference of evolution of these sequences results from their structural and functional peculiarities.
通过点杂交在不同生物体的DNA中检测到与小鼠基因组的短重复元件B1和B2同源的序列。B1和B2序列与鼠形啮齿动物和灵长类动物的DNA杂交效率最高。在所有其他研究的真核生物的DNA中也观察到了杂交现象，然而，这可能仅由与B1和B2序列存在短同源性引起。B1序列与灵长类动物DNA的有效杂交显然是由于它们的基因组中存在大量的Alu序列拷贝。克隆了一种能够与B2序列杂交的人类DNA重复序列。它被命名为HsB2序列。在人类基因组中大约有5000个这种序列的拷贝。为了估计同源程度，测量了B1和B2序列与啮齿动物和其他一些哺乳动物的DNA杂交体的解链温度。发现B2序列（而非B1）的同源程度与生物体的系统发育关系密切相关。也许，这些序列进化的差异源于它们的结构和功能特性。
