State key laboratory of food science and technology, Nanchang University, Nanchang 330047, PR China.
College of Basic Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330000, PR China.
J Immunol Methods. 2018 Nov;462:54-58. doi: 10.1016/j.jim.2018.08.008. Epub 2018 Aug 23.
Cronobacter spp. (previously Enterobacter sakazakii), an opportunistic food-borne pathogen, involved in fatal infections in neonates and infants. Contaminated powdered infant formula (PIF) was considered to be the uppermost infective resource. In the present study, a thermophilic helicase-dependent isothermal amplification (tHDA) based method combined with silica-coated magnetic nanoparticles (Si-MNPs) as a DNA separator for rapid and sensitive detection of Cronobacter has been developed. The whole detection process can be completed in <3 h, with a detection limit of 10 and 10 CFU/ml Cronobacter in pure culture and artificially contaminated PIF, respectively, with a great sensitivity (94%) and specificity (100%). Hence, this protocol might be useful for screening and monitoring the contamination of Cronobacter spp. in food industry, and helpful for avoiding the economic loss by retard of feedback of the contamination of pathogens in dairy products.
阪崎克罗诺杆菌（以前称为阪崎肠杆菌）是一种机会性食源性病原体，可导致新生儿和婴儿的致命感染。受污染的配方粉（PIF）被认为是最主要的感染源。本研究开发了一种基于嗜热解旋酶依赖性等温扩增（tHDA）的方法，并结合了硅涂层磁性纳米粒子（Si-MNPs）作为 DNA 分离剂，用于快速灵敏地检测阪崎克罗诺杆菌。整个检测过程可在 <3 小时内完成，在纯培养物和人工污染的 PIF 中，阪崎克罗诺杆菌的检测限分别为 10 和 10 CFU/ml，具有很高的灵敏度（94%）和特异性（100%）。因此，该方案可能有助于筛选和监测食品工业中阪崎克罗诺杆菌的污染情况，并有助于避免因乳制品中病原体污染反馈延迟而造成的经济损失。
