Department of Food Science and Technology, Yeungnam University, Gyeongsan-si, Gyeongsangbuk-do 38541, Republic of Korea.
Department of Energy and Material Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea.
Food Microbiol. 2018 Jun;72:23-30. doi: 10.1016/j.fm.2017.11.002. Epub 2017 Nov 8.
Cronobacter species are foodborne pathogens that can affect the human central nervous system. Survivors of Cronobacter infections often suffer from severe neurological impairments, including hydrocephalus, quadriplegia, and developmental delays in all ages, especially in infants and the immunocompromised. Moreover, Cronobacter species pose a high risk in powdered infant formula (PIF) because PIF is a major source of nutrition for infants worldwide. To develop a rapid and sensitive detection method for Cronobacter species in PIF, immunoliposomes and immunomagnetic nanoparticles were synthesized, after which an immunoliposome-based immunomagnetic concentration and separation assay was developed and applied to PIF for the detection of Cronobacter species. The detection limits of the developed assay were 5.9 × 10 ± 0.7-4.8 × 10 ± 0.2 CFU/mL for Cronobacter species in pure culture with no cross-reactivity with 13 other tested non-Cronobacter strains. Additionally, the developed assay could provide results in 3 h when the contaminated level was higher than 10 CFU/25 g PIF and in 9 h when the contaminated level was 10 CFU/25 g PIF. The developed immunoliposome-based immunomagnetic concentration and separation assay is rapid, sensitive, and simple and thus has great potential for use in the detection of Cronobacter species in PIF.
克罗诺杆菌属是食源性致病菌，可感染人类中枢神经系统。克罗诺杆菌属感染者通常患有严重的神经损伤，包括脑积水、四肢瘫痪和所有年龄段的发育迟缓，尤其是婴儿和免疫功能低下者。此外，克罗诺杆菌属在配方粉（PIF）中存在高风险，因为 PIF 是全球婴儿的主要营养来源。为了开发用于检测 PIF 中克罗诺杆菌属的快速、灵敏的检测方法，合成了免疫脂质体和免疫磁性纳米粒子，然后开发并应用基于免疫脂质体的免疫磁性浓缩和分离检测法来检测 PIF 中的克罗诺杆菌属。开发的检测法的检测限为 5.9×10±0.7-4.8×10±0.2 CFU/mL，用于纯培养物中的克罗诺杆菌属，与 13 种其他测试的非克罗诺杆菌属菌株无交叉反应。此外，当污染水平高于 10 CFU/25 g PIF 时，该检测法可在 3 h 内得出结果，当污染水平为 10 CFU/25 g PIF 时，该检测法可在 9 h 内得出结果。开发的基于免疫脂质体的免疫磁性浓缩和分离检测法快速、灵敏且简单，因此在检测 PIF 中的克罗诺杆菌属方面具有很大的应用潜力。
