College of Life Sciences of Longyan University, Longyan, 364012, Fujian, People's Republic of China.
Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan, 364012, Fujian, People's Republic of China.
Inflamm Res. 2018 Dec;67(11-12):903-911. doi: 10.1007/s00011-018-1183-2. Epub 2018 Aug 25.
Nuciferine, a major bioactive component from the lotus leaf, has been reported to have notable anti-inflammatory activities such as renal inflammation and acute lung injury in previous studies. Mastitis is one of the most prevalent diseases in the dairy cattle, which causes large economic losses for the dairy industry. However, the effects of nuciferine on lipopolysaccharide (LPS)-induced mastitis have not been reported.
Here, we investigated the anti-inflammatory effects of nuciferine on LPS-induced mastitis in mice and illuminated its potential mechanism on the TLR4-mediated signaling pathway in mouse mammary epithelial cells (mMECs). Histopathological changes and myeloperoxidase (MPO) activity assay showed that nuciferine treatment significantly alleviated the LPS-induced injury of mammary gland flocculus, inflammatory cells infiltration. qPCR and ELISA assays indicated that nuciferine dose-dependently reduced the levels of TNF-α and IL-1β, which indicated that nuciferine might have therapeutic effects on mastitis. Furthermore, nuciferine treatment significantly decreased the expression of TLR4 in a dose-dependent manner. Besides, nuciferine was also found to suppress LPS-induced NF-κB activation.
These findings indicate that nuciferine potently ameliorates LPS-induced mastitis by inhibition of the TLR4-NF-κB signaling pathway.
荷叶中的主要生物活性成分之一荷叶碱在以前的研究中被报道具有显著的抗炎活性，如肾炎症和急性肺损伤。乳腺炎是奶牛中最常见的疾病之一，给奶牛养殖业造成了巨大的经济损失。然而，荷叶碱对脂多糖（LPS）诱导的乳腺炎的影响尚未有报道。
在这里，我们研究了荷叶碱对 LPS 诱导的乳腺炎的抗炎作用，并阐明了其在 TLR4 介导的信号通路中对小鼠乳腺上皮细胞（mMECs）的潜在机制。组织病理学变化和髓过氧化物酶（MPO）活性测定表明，荷叶碱处理显著减轻了 LPS 诱导的乳腺小叶损伤和炎性细胞浸润。qPCR 和 ELISA 检测表明，荷叶碱呈剂量依赖性降低 TNF-α 和 IL-1β 的水平，表明荷叶碱可能对乳腺炎具有治疗作用。此外，荷叶碱处理还显著地以剂量依赖性方式降低 TLR4 的表达。此外，还发现荷叶碱能抑制 LPS 诱导的 NF-κB 激活。
这些发现表明，荷叶碱通过抑制 TLR4-NF-κB 信号通路，强力改善 LPS 诱导的乳腺炎。
