Chen Chu, Xu Guanhua, Yuan Kun, Sun Yuyu, Bao Guofeng, Xu Dawei, Cui Zhiming
Department of Spine Surgery, The Second Affiliated Hospital of Nantong University 6 Hai'er Alley North, Chongchuan District Nantong 226001 Jiangsu China
RSC Adv. 2018 Oct 1;8(59):33695-33701. doi: 10.1039/c8ra04809f. eCollection 2018 Sep 28.
It is recognized that facet joint osteoarthritis (FJOA) is commonly induced by the degeneration of articular cartilage of the facet joint. However, the specific pathological mechanisms underlying facet joint osteoarthritis has not yet been elucidated. To obtain the differential expression patterns and putative functions of long noncoding RNAs (lncRNAs) in FJOA, in the current study, we detected the expression levels of lncRNAs in patients with varying degrees of facet cartilage degeneration (control group: normal or mild facet cartilage degeneration; FJOA: moderate to severe facet cartilage degeneration) by RNA deep sequencing. Differentially expressed lncRNAs were screened and the accuracy of sequencing data was further validated by using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Target genes of differentially expressed lncRNAs were predicted by antisense and/or -regulatory module prediction. Predicted target genes were further analyzed by Gene Ontology (GO) and Kyoto Enrichment of Genes and Genomes pathway analysis (KEGG) to discover enriched cellular component, molecular function, biological process, and signaling pathways. Our results provided a general view of the expression changes of lncRNAs in FJOA and thus might facilitate the illumination of the underlying mechanisms of FJOA.
人们认识到，小关节骨关节炎（FJOA）通常由小关节的关节软骨退变引起。然而，小关节骨关节炎潜在的具体病理机制尚未阐明。为了获得长链非编码RNA（lncRNAs）在FJOA中的差异表达模式及潜在功能，在本研究中，我们通过RNA深度测序检测了不同程度小关节软骨退变患者（对照组：正常或轻度小关节软骨退变；FJOA组：中度至重度小关节软骨退变）lncRNAs的表达水平。筛选出差异表达的lncRNAs，并通过定量逆转录-聚合酶链反应（qRT-PCR）进一步验证测序数据的准确性。通过反义及/或调控模块预测来预测差异表达lncRNAs的靶基因。对预测的靶基因通过基因本体（GO）和京都基因与基因组百科全书通路分析（KEGG）进一步分析，以发现富集的细胞成分、分子功能、生物学过程和信号通路。我们的结果提供了FJOA中lncRNAs表达变化的总体情况，因此可能有助于阐明FJOA的潜在机制。
