Department of Biochemistry, Central University of Rajasthan, Bandarsindri, Ajmer, India.
J Cell Biochem. 2018 Dec;119(12):10314-10326. doi: 10.1002/jcb.27373. Epub 2018 Aug 26.
Acinetobacter baumannii has emerged as a hospital-acquired pathogen and has spread in the hospital settings, leading to enhanced nosocomial outbreaks associated with high death rates. Therefore, the aim of the current study is to determine the effective concentration of disinfectants like sodium hypochlorite, hydrogen peroxide, and chlorine dioxide, against multidrug-resistant (MDR) strains of A. baumannii. In this study, we have investigated the effect of disinfectants on different MDR strains i.e. RS307, RS6694, RS7434, RS10953, RS122, and sensitive strain ATCC-19606 of A. baumannii, via differential growth curves analysis, disc diffusion assay, estimation of reactive oxygen species (ROS), lipid peroxidation, and protein carbonylation. All the results collectively showed that 1% sodium hypochlorite (P value < 0.0027), 2.5% hydrogen peroxide (P value = 0.0032), and 10 mM (P value = 0.017) chlorine dioxide significantly inhibit the growth of MDR strains of A. baumannii. A significant increase in the ROS generations, altered lipid peroxidation, and a decrease in protein carbonylation was also observed after treatment with disinfectants, which confirmed its ROS-dependent damage mechanism. These disinfectants also enhance the membrane leakage of reducing sugar, protein, and DNA. The current study highlights and recommends the use of 2.5% hydrogen peroxide to control the MDR strains of A. baumannii in the hospital setup. Therefore, the present results will help in selecting concentrations of different disinfectants for regular use in hospital setups to eradicate the multidrug-resistant A. baumannii from the hospital setup.
鲍曼不动杆菌已成为医院获得性病原体，并在医院环境中传播，导致与高死亡率相关的医院感染爆发增加。因此，本研究旨在确定次氯酸钠、过氧化氢和二氧化氯等消毒剂对多重耐药（MDR）鲍曼不动杆菌菌株的有效浓度。在这项研究中，我们通过差异生长曲线分析、圆盘扩散测定、活性氧（ROS）、脂质过氧化和蛋白质羰基化的估计，研究了消毒剂对不同 MDR 菌株（即 RS307、RS6694、RS7434、RS10953、RS122 和敏感菌株 ATCC-19606）的影响。所有结果均表明，1%次氯酸钠（P 值<0.0027）、2.5%过氧化氢（P 值=0.0032）和 10mM 二氧化氯（P 值=0.017）显著抑制了 MDR 鲍曼不动杆菌菌株的生长。在用消毒剂处理后，还观察到 ROS 生成显著增加、脂质过氧化改变和蛋白质羰基化减少，这证实了其 ROS 依赖性损伤机制。这些消毒剂还增强了还原糖、蛋白质和 DNA 的膜泄漏。本研究强调并推荐在医院环境中使用 2.5%过氧化氢来控制 MDR 鲍曼不动杆菌菌株。因此，目前的结果将有助于选择不同消毒剂的浓度，以便在医院环境中定期使用，从而从医院环境中消除多重耐药的鲍曼不动杆菌。
