Department of Biochemistry, Central University of Rajasthan, Ajmer, India.
PLoS One. 2019 Oct 29;14(10):e0224107. doi: 10.1371/journal.pone.0224107. eCollection 2019.
Acinetobacter baumannii causes hospital-acquired infections, especially in those with impaired immune function. Biocides or disinfectants are widely used antibacterial agents used to eradicate the effect of A. baumannii on inanimate objects and health care environments. In the current study, the antimicrobial activity of chlorhexidine has been investigated against carbapenem-resistant (RS-307, RS-7434, RS-6694, and RS-122), and sensitive (ATCC-19606 and RS-10953) strains of A. baumannii. We have determined growth kinetics, antimicrobial susceptibility, ROS production, lipid peroxidation, cell viability using flow cytometry assay (FACS), and membrane integrity by scanning electron microscope (SEM). The effect of chlorhexidine on the bacterial membrane has also been investigated using Fourier transform infrared (FTIR) spectroscopy. The present study showed that 32μg/ml chlorhexidine treatment results in the decreased bacterial growth, CFU count and cell viability. The antibacterial activity of chlorhexidine is due to the elevated ROS production and higher lipid peroxidation. These biochemical changes result in the membrane damage and alteration in the membrane proteins, phospholipids, carbohydrates, nucleic acids as evident from the FTIR and SEM data. Therefore, chlorhexidine has the potential to be used in the hospital setups to remove the spread of A. baumannii.
鲍曼不动杆菌会引起医院获得性感染，尤其是在免疫功能受损的人群中。消毒剂被广泛用作抗菌剂，用于消除鲍曼不动杆菌对无生命物体和医疗保健环境的影响。在目前的研究中，研究了洗必泰对碳青霉烯类耐药（RS-307、RS-7434、RS-6694 和 RS-122）和敏感（ATCC-19606 和 RS-10953）株鲍曼不动杆菌的抗菌活性。我们测定了生长动力学、抗菌药敏性、ROS 产生、脂质过氧化、使用流式细胞术（FACS）测定的细胞活力以及扫描电子显微镜（SEM）测定的细胞膜完整性。还使用傅里叶变换红外（FTIR）光谱研究了洗必泰对细菌膜的影响。本研究表明，32μg/ml 洗必泰处理会导致细菌生长、CFU 计数和细胞活力下降。洗必泰的抗菌活性是由于 ROS 产生增加和脂质过氧化增强所致。这些生化变化导致膜损伤和膜蛋白、磷脂、碳水化合物、核酸的改变，这从 FTIR 和 SEM 数据中可以明显看出。因此，洗必泰有可能在医院环境中用于消除鲍曼不动杆菌的传播。
