Life Science School of East, China Normal University, Dongchuan Road 500, Shanghai, 200241, China.
Shanghai Institute of Plant Physiology and Ecology of Chinese Academy of Science, Fenglin Road 300, Shanghai, 200032, China.
New Phytol. 2019 Jan;221(2):1160-1171. doi: 10.1111/nph.15394. Epub 2018 Aug 26.
The moss Sphagnum (peat moss) is ecologically and economically important. There is a paucity of physiological and developmental studies on Sphagnum because of the lack of an axenic culture system for its whole life cycle. A culture system has been established for the Sphagnum gametophore, but not the protonema (juvenile vegetative stage after spore germination). Therefore, the aim of this study was to develop a protonema culture system for Sphagnum. Sphagnum squarrosum gametophore tissue was disrupted and then cultured in liquid Knop medium. The regeneration of protonemata from the gametophore fragments was analyzed in detail by microscopy. We observed a developmental balance between filamentous and thalloid protonemata, and growth competition between the thalloid protonema and the gametophore. On the basis of these findings, we established a relatively stable peat moss protonema proliferation method. Using this method, all the developmental stages of peat moss vegetative growth could be obtained through differentiation or regeneration. The method can provide abundant homogeneous Sphagnum materials at desired stages for physiological and developmental studies, and will be useful for large-scale Sphagnum vegetative proliferation. The regeneration analysis method will be useful for establishing protonema proliferation systems for other mosses.
藓类植物（泥炭藓）在生态和经济上都很重要。由于缺乏其整个生命周期的无菌培养体系，因此对藓类植物的生理和发育研究很少。已经建立了藓类植物配子体的培养体系，但没有建立原丝体（孢子萌发后的幼龄营养阶段）的培养体系。因此，本研究旨在为藓类植物建立原丝体培养体系。将藓类植物 squarrosum 的配子体组织打碎，然后在液体 Knop 培养基中培养。通过显微镜详细分析了从配子体片段再生原丝体的情况。我们观察到丝状和叶状原丝体之间存在发育平衡，以及叶状原丝体和配子体之间的生长竞争。在此基础上，我们建立了一种相对稳定的泥炭藓原丝体增殖方法。使用该方法，可以通过分化或再生获得藓类植物营养生长的所有发育阶段。该方法可为生理和发育研究提供丰富的同质藓类植物材料，并有助于大规模的藓类植物营养繁殖。再生分析方法将有助于建立其他藓类植物原丝体增殖系统。
