School of Public Health, Xuzhou Medical University, Xuzhou 221000, Jiangsu, China.
Biomed Environ Sci. 2018 Jul;31(7):499-506. doi: 10.3967/bes2018.067.
To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes (HOKs), and to clarify whether the mechanisms involve transforming growth factor-β (TGF-β) signaling.
HOKs were transfected with the plasmid pEGFP-N3-YOD1 containing YOD1. The mRNA levels of YOD1 and TGF-β were determined by qPCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively.
The mRNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mRNA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels.
YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.
研究 YOD1 过表达对人口腔角质细胞（HOK）增殖和迁移的影响，并阐明其机制是否涉及转化生长因子-β（TGF-β）信号通路。
用含有 YOD1 的 pEGFP-N3-YOD1 质粒转染 HOK。用 qPCR 测定 YOD1 和 TGF-β 的 mRNA 水平。用 Western blot 测定 YOD1、TGF-β、Smad2/3、Smad4 和磷酸化 Smad2/3 的蛋白表达。用细胞计数试剂盒-8 法和划痕愈合试验分别评估细胞增殖和迁移。
转染 YOD1 的 HOK 中 YOD1 的 mRNA 和蛋白水平均升高。YOD1 过表达显著增强了 HOK 的迁移能力。YOD1 过表达增加了 TGF-β3 的 mRNA 和蛋白水平。转染 YOD1 的 HOK 中磷酸化 Smad2/3 水平升高。
YOD1 过表达通过促进 TGF-β3 信号通路增强细胞迁移，这可能在唇腭裂形成中发挥重要作用。YOD1 突变可能导致 TGF-β3 信号异常，细胞迁移减少，导致 NSCLP。
