Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, 130021, China.
School of Dentistry, Lanzhou University, Lanzhou, China.
Arch Oral Biol. 2018 Nov;95:187-194. doi: 10.1016/j.archoralbio.2018.08.004. Epub 2018 Aug 15.
The width of keratinized mucosa plays an important role in esthetic and functional outcomes of dental implants. Lack of keratinized mucosa may lead to poor oral hygiene and greater soft-tissue recession. This study aimed at assessing the potential of quercetin in promoting human oral keratinocyte (HOK) proliferation and re-epithelialization in vitro.
HOK were detected in the absence or presence of test substances. The Cell Counting Kit-8 was used to assess cell viability and proliferation capacity. Re-epithelization was assessed using a keratinocyte monolayer scratch assay. Cell migration was monitored via Transwell chambers. Porphyromonas gingivalis lipopolysaccharide was used to stimulate keratinocytes for mimicking the inflammatory situation. mRNA expression of inflammatory cytokines (interleukin-1beta, IL-1β and tumor necrosis factor alpha, TNF-α), cell adhesion molecules (Integrin-α6, Integrin-β4), and growth factors (transforming growth factor beta 1,TGF-β1 and transforming growth factor beta 3, TGF-β3) were estimated using RT-qPCR. Protein contents of TGF-β1 and TGF-β3 were investigated by enzyme-linked immunosorbent assay.
Multiplex analysis revealed that quercetin enhances HOK proliferation via an upregulation of adhesion molecules (Integrin-α6β4). Additionally, re-epithelialization rate was significantly greater in the presence of quercetin than in the control (P < 0.01). Furthermore, 20 μM of quercetin increases both mRNA and protein levels of TGF-β3 under basal and wound conditions without affecting TGF-β1 production. Expressions of pro-inflammatory cytokines were downregulated by quercetin treatment.
Quercetin promotes HOKs proliferation and oral re-epithelialization in vitro.
角化黏膜的宽度在牙种植体的美学和功能效果中起着重要作用。缺乏角化黏膜可能导致口腔卫生不良和软组织进一步退缩。本研究旨在评估槲皮素在体外促进人口腔角质细胞（HOK）增殖和再上皮化的潜力。
在不存在或存在测试物质的情况下检测 HOK。使用细胞计数试剂盒-8 评估细胞活力和增殖能力。通过角质细胞单层划痕试验评估再上皮化。通过 Transwell 室监测细胞迁移。使用牙龈卟啉单胞菌脂多糖刺激角质细胞模拟炎症情况。使用 RT-qPCR 估计炎症细胞因子（白细胞介素 1β，IL-1β 和肿瘤坏死因子 α，TNF-α）、细胞粘附分子（整合素-α6、整合素-β4）和生长因子（转化生长因子β 1、TGF-β1 和转化生长因子β 3、TGF-β3）的 mRNA 表达。通过酶联免疫吸附试验研究 TGF-β1 和 TGF-β3 的蛋白含量。
多重分析显示，槲皮素通过上调粘附分子（整合素-α6β4）来增强 HOK 增殖。此外，与对照组相比，存在槲皮素时再上皮化率显著更高（P<0.01）。此外，20 μM 的槲皮素在基础和伤口条件下均增加 TGF-β3 的 mRNA 和蛋白水平，而不影响 TGF-β1 的产生。槲皮素处理可下调促炎细胞因子的表达。
槲皮素促进体外 HOKs 的增殖和口腔再上皮化。
