[新疆地区汉族和维吾尔族人群中WNT10A基因rs10177996多态性的分布]

[Distribution of WNT10A gene rs10177996 polymorphism between Han and Uygur populations in Xinjiang area].

作者信息

Liu Jia-Lin, Zhao Chun-Ping, Zhang Jia-Yu, Han Xiang-Zhen, Ge Shu-Peng, DU Li-Juan, He Hui-Yu

机构信息

Department of Prosthodontics, the First Affiliated Hospital of Xinjiang Medical University. Urumqi 830054, China. E-mail:

出版信息

Shanghai Kou Qiang Yi Xue. 2018 Apr;27(2):164-169.

PMID:30146643

Abstract

PURPOSE

To explore the characteristics of distribution of WNT10A gene rs10177996 polymorphism between Han and Uygur populations in Xinjiang area.

METHODS

A cross-sectional survey on 154 Han individuals in Urumqi area and 134 Uygur individuals in Kashgar area was performed. Buccal epithelial cells were harvested using Cotton swab scraping, and DNA was extracted by special kit. After screening, the corresponding SNP segments of qualified samples were propagated by PCR. Dideoxy-mediated chain termination method was used for gene sequencing, and then, genotyping was conducted with corresponding software. Statistical analysis of genetic data was performed by SPSS 23.0 software package.

RESULTS

Among Uygur nationality in Kashgar area, the frequencies of CC, CT, TT genetypes in rs10177996 were 8.21%, 30.60% and 61.19%, respectively. The allele frequency of C was 23.51% and T was 76.49%. Among Han nationality in Urumqi area, the frequencies on CC, CT, TT genetypes of rs10177996 were 9.74%, 43.51% and 46.75%, respectively. The allele frequency of C was 31.49% and T was 68.51%. When compared with Han nationality, the frequency of TT was significantly higher in Uygur nationality(P=0.046). When compared with European, the frequency of TT was significantly lower in Uygur nationality (P=0.05). When compared with European, the frequency of TT was significantly lower in Han nationality(P＜0.01). Compared with European, the distribution on C allele frequency was significantly higher, the distribution on T allele frequency was significantly lower in Han nationality (P=0.033). However, there was no significant difference between Han nationality in Urumqi area and Uygur nationality in Kashgar area (P＞0.05), and, between Uygur nationality in Kashgar area and European (P＞0.05). Meanwhile, there was no significant difference in gender between Uygur nationality in Kashgar area and Han nationality in Urumqi area (P＞0.05).

CONCLUSIONS

The distributions of WNT10A gene rs10177996 SNP among Han nationality in Urumqi area, Uygur nationality in Kashgar area and the reported European population are obviously different.

摘要

目的

探讨新疆地区汉族和维吾尔族人群中WNT10A基因rs10177996多态性的分布特征。

方法

对乌鲁木齐地区154名汉族个体和喀什地区134名维吾尔族个体进行横断面调查。用棉签刮取法采集颊黏膜上皮细胞，采用专用试剂盒提取DNA。筛选后，对合格样本的相应SNP片段进行PCR扩增。采用双脱氧介导的链终止法进行基因测序，然后用相应软件进行基因分型。采用SPSS 23.0软件包对遗传数据进行统计分析。

结果

喀什地区维吾尔族中，rs10177996的CC、CT、TT基因型频率分别为8.21%、30.60%和61.19%。C等位基因频率为23.51%，T等位基因频率为76.49%。乌鲁木齐地区汉族中，rs10177996的CC、CT、TT基因型频率分别为9.74%、43.51%和46.75%。C等位基因频率为31.49%，T等位基因频率为68.51%。与汉族相比，维吾尔族中TT基因型频率显著更高（P=0.046）。与欧洲人相比，维吾尔族中TT基因型频率显著更低（P=0.05）。与欧洲人相比，汉族中TT基因型频率显著更低（P＜0.01）。与欧洲人相比，汉族中C等位基因频率分布显著更高，T等位基因频率分布显著更低（P=0.033）。然而，乌鲁木齐地区汉族与喀什地区维吾尔族之间无显著差异（P＞0.05），喀什地区维吾尔族与欧洲人之间也无显著差异（P＞0.05）。同时，喀什地区维吾尔族与乌鲁木齐地区汉族在性别上无显著差异（P＞0.05）。