Bai Xu-Hua, Lan Jiong-Cai, Gong Xiao-Yan, Cui Li, Zhou Hua-You
Blood Center of Xinjiang Production & Construction Corps, Urumqi 830002, Xinjiang Uygur Autonomous Region, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Oct;18(5):1335-7.
This study was aimed to investigate the characteristics of RHCE genotyping of Xinjiang Uygur nationality population in China. Primers for detecting RHCE genes were designed according to the references, 89 Uygur nationality RhD-negative samples, 233 Han nationality RhD-negative samples and 109 Han nationality RhD-positive samples were detected by sequence-specific primer-polymerase chain reaction (SSP-PCR) for RHCE genotyping. All above-mentioned samples were unrelated. The results indicated that RHE/e genotyping results were consistent with the serological test results in the samples of Uygur and Han nationality, regardless of the RhD-negative samples or the RhD-positive samples. The RHC/c genotyping results from 89 RhD-negative samples of Uygur nationality were consistent with serological test results. However, total error of RHC/c genotyping from 233 RhD-negative and 109 RhD-positive samples of Han nationality was 5.05%. In conclusion, this method of RHCE genotyping is suitable for the analysis of the RHE/e genotyping of Uygur nationality, no erroneous RHC/c genotyping of Uygur nationality was found in this study, but this method needs to be further studied.
本研究旨在探讨中国新疆维吾尔族人群RHCE基因分型特征。参照文献设计检测RHCE基因的引物，采用序列特异性引物聚合酶链反应（SSP-PCR）对89例维吾尔族RhD阴性样本、233例汉族RhD阴性样本和109例汉族RhD阳性样本进行RHCE基因分型检测。上述所有样本均无亲缘关系。结果表明，维吾尔族和汉族样本中，无论RhD阴性样本还是RhD阳性样本，RHE/e基因分型结果均与血清学检测结果一致。89例维吾尔族RhD阴性样本的RHC/c基因分型结果与血清学检测结果一致。然而，233例汉族RhD阴性样本和109例汉族RhD阳性样本的RHC/c基因分型总错误率为5.05%。综上所述，该RHCE基因分型方法适用于维吾尔族RHE/e基因分型分析，本研究未发现维吾尔族RHC/c基因分型有误，但该方法有待进一步研究。
