在酵母中表达的莫洛尼鼠肉瘤病毒编码的p37mos具有蛋白激酶活性。
Moloney murine sarcoma virus encoded p37mos expressed in yeast has protein kinase activity.
作者信息
Singh B, Wittenberg C, Reed S I, Arlinghaus R B
出版信息
Virology. 1986 Jul 30;152(2):502-6. doi: 10.1016/0042-6822(86)90156-x.
PMID:3014729
Abstract
We describe the expression of the Moloney murine sarcoma virus env-mos protein (p37mos) in yeast under the control of the yeast GAL1 promoter. Consistent with our previous results concerning the p37mos protein kinase made in virus infected mouse cells, p37mos produced in yeast possesses autophosphorylation activity in an immune complex kinase assay using anti-mos (37-35) serum.
摘要
我们描述了莫洛尼鼠肉瘤病毒env-mos蛋白（p37mos）在酵母GAL1启动子控制下的表达情况。与我们之前关于病毒感染小鼠细胞中产生的p37mos蛋白激酶的结果一致，酵母中产生的p37mos在使用抗mos（37-35）血清的免疫复合物激酶测定中具有自磷酸化活性。
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