小鼠Mos原癌基因产物在卵子发生过程中存在并发挥作用。

Mouse Mos protooncogene product is present and functions during oogenesis.

作者信息

Paules R S, Buccione R, Moschel R C, Vande Woude G F, Eppig J J

机构信息

BRI-Basic Research Program, National Cancer Institute-Frederick Cancer Research Facility, MD 21701.

出版信息

Proc Natl Acad Sci U S A. 1989 Jul;86(14):5395-9. doi: 10.1073/pnas.86.14.5395.

We have identified the mouse Mos-encoded protein product, p39mos, in maturing mouse oocytes and have shown that it is indistinguishable from the product expressed in Mos-transformed NIH 3T3 cells. p39mos is detected in oocytes arrested in the first meiotic prophase, during germinal-vesicle breakdown, metaphase I, anaphase I, and in ovulated eggs. We show that microinjection of three different Mos antisense (but not sense) oligodeoxyribonucleotides into germinal vesicle-stage oocytes prevents first polar-body emission and therefore interrupted the normal progression of meiosis. These results show that in mouse oocytes, as in the amphibian Xenopus [Sagata, N., Oskarsson, M., Copeland, T., Brumbaugh, J. & Vande Woude, G.F. (1988) Nature (London) 335, 519-525], the product of Mos is necessary for normal meiotic maturation.

我们已在成熟的小鼠卵母细胞中鉴定出小鼠Mos编码的蛋白产物p39mos，并表明它与在Mos转化的NIH 3T3细胞中表达的产物没有区别。在第一次减数分裂前期停滞的卵母细胞、生发泡破裂期、中期I、后期I以及排卵后的卵子中均检测到p39mos。我们发现，将三种不同的Mos反义（而非正义）寡脱氧核糖核苷酸显微注射到生发泡期卵母细胞中可阻止第一极体排出，从而中断减数分裂的正常进程。这些结果表明，在小鼠卵母细胞中，如同在两栖动物非洲爪蟾中一样[相田，N.，奥斯卡松，M.，科普兰，T.，布伦博，J. & 范德伍德，G.F.（1988年）《自然》（伦敦）335卷，519 - 525页]，Mos的产物对于正常的减数分裂成熟是必需的。