Etingof R N, Kalinina S N, Dumler I L
Vision Res. 1986;26(3):415-23. doi: 10.1016/0042-6989(86)90184-7.
Monospecific precipitating antibodies raised to phosphodiesterase, its protein inhibitor and GTP-binding proteins of bovine retina photoreceptor membranes were obtained. The characterization of the antibodies was carried out by immunochemical methods and according to their functional properties as determined by their effect on enzyme activities. The antibodies were used to study the distribution of immunolike proteins in different animal retinas and to purify the inhibitor protein by the method of immunoaffinity chromatography.
制备了针对牛视网膜光感受器膜磷酸二酯酶、其蛋白抑制剂和GTP结合蛋白的单特异性沉淀抗体。通过免疫化学方法并根据其对酶活性的影响所确定的功能特性对抗体进行了表征。这些抗体被用于研究不同动物视网膜中免疫样蛋白的分布，并通过免疫亲和层析法纯化抑制剂蛋白。
