Kalinina S N
Biokhimiia. 1985 Dec;50(12):2063-70.
It was found that antibodies specifically raised against GTP-binding proteins of bovine retina photoreceptor membranes block the inhibiting effect of estradiol on phosphodiesterase from human and rat uterus cytosol and prevent the combined effect of catecholamines and guanylyl-5'-imidodiphosphate on rat skeletal muscle adenylate cyclase. Using double radial immunodiffusion, it was demonstrated that the antibodies form a precipitating complex with purified tubulin from bovine brain as well as with retinal preparations obtained from the visual organs of ox, pig, rat, frog, some fish species and one reptile species. No appearance of a precipitation band was observed, when retinal preparations of invertebrates (squid, octopus) were used as antigens. The antibodies interacted with the alpha- and beta-subunits of GTP-binding proteins of bovine retinal photoreceptor membranes.
研究发现，针对牛视网膜光感受器膜的GTP结合蛋白特异性产生的抗体，可阻断雌二醇对人及大鼠子宫胞质溶胶中磷酸二酯酶的抑制作用，并阻止儿茶酚胺和鸟苷-5'-亚氨二磷酸对大鼠骨骼肌腺苷酸环化酶的联合作用。采用双向辐射免疫扩散法表明，这些抗体与来自牛脑的纯化微管蛋白以及从牛、猪、大鼠、青蛙、某些鱼类和一种爬行动物的视觉器官获得的视网膜制剂形成沉淀复合物。当使用无脊椎动物（鱿鱼、章鱼）的视网膜制剂作为抗原时，未观察到沉淀带的出现。这些抗体与牛视网膜光感受器膜的GTP结合蛋白的α和β亚基相互作用。
