Wortmann R L, Ryan L M, Karas B, McCarthy D J
Adv Exp Med Biol. 1986;195 Pt A:153-6. doi: 10.1007/978-1-4684-5104-7_23.
Articular cartilage contains any ectoenzyme activity, NTP-PPH, which is capable of generating PPi from NTP substrates. The PPi generated is from the cleavage of the alpha-beta pyrophosphate bond of NTP and does not result from the effects of NTP catabolites. NTP-PPH activity is expressed on human skin fibroblasts in culture and is significantly increased in subjects with CPPD deposition. In addition, cultured fibroblasts from subjects with CPPD disease have higher intracellular PPi concentrations compared to cells from normals and patients with OA. These results support the hypothesis that alterations in PPi metabolism provide the metabolic basis for CPPD deposition.
关节软骨含有一种外切酶活性，即NTP - PPH，它能够从NTP底物生成焦磷酸（PPi）。所生成的PPi来自NTP的α - β焦磷酸键的断裂，而非NTP分解代谢产物的作用。NTP - PPH活性在培养的人皮肤成纤维细胞中表达，并且在患有焦磷酸钙沉积症（CPPD）的受试者中显著增加。此外，与正常人及骨关节炎（OA）患者的细胞相比，来自CPPD疾病患者的培养成纤维细胞具有更高的细胞内PPi浓度。这些结果支持了这样一种假说，即PPi代谢的改变为CPPD沉积提供了代谢基础。
