Fawzy El-Sayed K, Graetz C, Köhnlein T, Mekhemar M, Dörfer C
Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts-Universität zu Kiel, Arnold-Heller-Str. 3, Haus 26, 24105 Kiel, Germany,
Med Oral Patol Oral Cir Bucal. 2018 Sep 1;23(5):e569-e578. doi: 10.4317/medoral.22661.
Aggregatibacter-actinomycetemcomitans (A.actinomycetemcomitans) are strongly associated with localized-aggressive-periodontitis (LAgP). The study's aim was to test for the first time the effect of total sonicated A.actinomycetemcomitans-bacterial-fragments on gingival mesenchymal stem/progenitor cells' (G-MSCs) proliferation and regenerative gene expression in-vitro.
G-MSCs were isolated, characterized, expanded and stimulated by total sonicated A.actinomycetemcomitans-bacterial-fragments (0 (negative-control), 15, 60, 120 and 240µg/ml; serovar-b; n=6/group). Cellular proliferation and NF-κβ (NFKB1), Alkaline Phosphatase (ALPL), Collagen-I (COL1A1), Collagen-III (COL3A1), Osteonectin (SPARC) and Osteopontin (SPP1) m-RNA expression were assessed via reverse-transcription-polymerase-chain-reaction (RT-PCR) at 24, 48 and 72 hours and CFUs-ability evaluated at twelve days.
G-MSCs demonstrated stem/progenitor cells' characteristics. A.actinomycetemcomitans-bacterial-fragments (up to 72 hours) resulted in marked G-MSCs' proliferation over-time (p<0.001) and elevated NFKB1 (p=0.017), COL1A1 (p=0.025), SPARC (p=0.025), decreased ALPL (p=0.017), with no significant differences for COL3A1 and SPP1 expression or stimulation times (p>0.05; Friedman-test). Longer-term stimulation for twelve days reduced G-MSCs' CFUs.
Sonicated A.actinomycetemcomitans-bacterial-fragments' exert beneficial short-term effects on G-MSCs' proliferative and non-mineralized tissue forming aptitude. Results shed new light on the importance of periodontal treatment for LAgP patients, using power driven sonic/ultrasonic devices, which, in addition to reducing the subgingival microbial load, produces cell-stimulatory A.actinomycetemcomitans-bacterial-fragments, with positive attributes on tissue reparative/regenerative responses of tissue resident stem/progenitor cells in their niche.
伴放线聚集杆菌（A.actinomycetemcomitans）与局限性侵袭性牙周炎（LAgP）密切相关。本研究旨在首次测试超声处理后的伴放线聚集杆菌全菌片段对牙龈间充质干/祖细胞（G-MSCs）体外增殖及再生相关基因表达的影响。
分离、鉴定、扩增G-MSCs，并使用超声处理后的伴放线聚集杆菌全菌片段（0（阴性对照）、15、60、120和240μg/ml；b血清型；每组n = 6）对其进行刺激。在24、48和72小时通过逆转录聚合酶链反应（RT-PCR）评估细胞增殖以及NF-κβ（NFKB1）、碱性磷酸酶（ALPL）、I型胶原蛋白（COL1A1）、III型胶原蛋白（COL3A1）、骨连接蛋白（SPARC）和骨桥蛋白（SPP1）的mRNA表达，并在第12天评估集落形成单位（CFUs）能力。
G-MSCs表现出干/祖细胞的特征。伴放线聚集杆菌全菌片段（长达72小时）导致G-MSCs随时间显著增殖（p<0.001），NFKB1升高（p = 0.017）、COL1A1升高（p = 0.025）、SPARC升高（p = 0.025），ALPL降低（p = 0.017），COL3A1和SPP1的表达及刺激时间无显著差异（p>0.05；Friedman检验）。为期12天的长期刺激降低了G-MSCs的CFUs。
超声处理后的伴放线聚集杆菌全菌片段对G-MSCs的增殖及形成非微小组织的能力具有有益的短期影响。研究结果为LAgP患者的牙周治疗的重要性提供了新的思路，使用动力驱动的超声设备，除了减少龈下微生物负荷外，还能产生对组织驻留干/祖细胞在其微环境中的组织修复/再生反应具有积极作用的细胞刺激伴放线聚集杆菌全菌片段。
