Porter Caryn, Ennamorati Maria, Jain Nitya
Center for Computational and Integrative Biology, Massachusetts General Hospital;
Mucosal Immunology and Biology Research Center, Massachusetts General Hospital.
J Vis Exp. 2018 Aug 10(138):57929. doi: 10.3791/57929.
Enteric bacterial communities are established early in life and influence immune cell development and function. The neonatal microbiota is susceptible to numerous external influences including antibiotics use and diet, which impacts susceptibility to autoimmune and inflammatory diseases. Disorders such as Inflammatory Bowel Disease (IBD) are characterized by a massive influx of immune cells to the intestines. However, immune cells conditioned by the microbiota may additionally emigrate out of the intestines to influence immune responses at extra-intestinal sites. Thus, there is a need to identify and characterize cells that may carry microbial messages from the intestines to distal sites. Here, we describe a method to label cells in the colon of newborn mice in vivo that enables their identification at extra-intestinal sites after migration.
肠道细菌群落早在生命早期就已建立，并影响免疫细胞的发育和功能。新生儿微生物群易受多种外部因素影响，包括抗生素使用和饮食，这会影响自身免疫性疾病和炎症性疾病的易感性。诸如炎症性肠病（IBD）等疾病的特征是大量免疫细胞涌入肠道。然而，受微生物群调节的免疫细胞可能还会迁移出肠道，以影响肠外部位的免疫反应。因此，有必要识别和表征可能将微生物信息从肠道传递到远端部位的细胞。在此，我们描述了一种在新生小鼠体内标记结肠细胞的方法，该方法能够在细胞迁移后在肠外部位对其进行识别。
