Deparment of Medical Science, College of Medical Science, SoonChunHyang University, Asan, Chungnam, Korea.
Department of Medical Biotechnology, College of Medical Science, SoonChunHyang University, Asan, Chungnam, Korea.
PLoS One. 2018 Aug 27;13(8):e0202933. doi: 10.1371/journal.pone.0202933. eCollection 2018.
In this study, we investigated the anti-microbial, anti-inflammatory, and anti-lipogenic effects of hemp (Cannabis sativa L.) seed hexane extracts, focusing on the Propionibacterium acnes-triggered inflammation and lipogenesis. Hemp seed hexane extracts (HSHE) showed anti-microbial activity against P. acnes. The expression of iNOS, COX-2, and the subsequent production of nitric oxide and prostaglandin increased after infection of P. acnes in HaCaT cells, however, upon treating with HSHE, their expressions were reduced. P. acnes-induced expressions of IL-1β and IL-8 were also reduced. HSHE exerted anti-inflammatory effects by regulating NF-κB and MAPKs signaling and blunting the translocation of p-NF-κB to the nucleus in P. acnes-stimulated HaCaT cells. Moreover, P. acnes-induced phosphorylation of ERK and JNK, and their downstream targets c-Fos and c-Jun, was also inhibited by HSHE. In addition, the transactivation of AP-1 induced by P. acnes infection was also downregulated by HSHE. Notably, HSHE regulated inflammation and lipid biosynthesis via regulating AMPK and AKT/FoxO1 signaling in IGF-1-induced inflammation and lipogenesis of sebocytes. In addition, HSHE inhibited 5-lipoxygenase level and P. acnes-induced MMP-9 activity, and promoted collagen biosynthesis in vitro. Thus, HSHE could be utilized to treat acne vulgaris, through its anti-microbial, anti-inflammatory, anti-lipogenic, and collagen-promoting properties.
在这项研究中，我们研究了大麻（Cannabis sativa L.）种子正己烷提取物的抗微生物、抗炎和抗脂肪生成作用，重点研究了痤疮丙酸杆菌触发的炎症和脂肪生成。大麻种子正己烷提取物（HSHE）对痤疮丙酸杆菌具有抗微生物活性。痤疮丙酸杆菌感染 HaCaT 细胞后，iNOS、COX-2 的表达以及随后的一氧化氮和前列腺素的产生增加，但用 HSHE 处理后，其表达减少。痤疮丙酸杆菌诱导的 IL-1β 和 IL-8 的表达也减少。HSHE 通过调节 NF-κB 和 MAPKs 信号通路发挥抗炎作用，并阻止 P. acnes 刺激的 HaCaT 细胞中 p-NF-κB 向核内易位。此外，HSHE 还抑制了痤疮丙酸杆菌诱导的 ERK 和 JNK 的磷酸化及其下游靶标 c-Fos 和 c-Jun。此外，由痤疮丙酸杆菌感染诱导的 AP-1 的转录激活也被 HSHE 下调。值得注意的是，HSHE 通过调节 IGF-1 诱导的炎症和脂肪生成中 AMPK 和 AKT/FoxO1 信号通路来调节炎症和脂质生物合成。此外，HSHE 抑制了 5-脂氧合酶水平和痤疮丙酸杆菌诱导的 MMP-9 活性，并促进了体外胶原蛋白的合成。因此，HSHE 可以通过其抗微生物、抗炎、抗脂肪生成和促进胶原蛋白生成的特性用于治疗寻常痤疮。
