Castagnetta L, Granata O M, Lo Casto M, D'Agostino G, Mitchell F, O'Hare M J
Ann N Y Acad Sci. 1986;464:316-30. doi: 10.1111/j.1749-6632.1986.tb16012.x.
This paper presents a short review of the results obtained to date in our laboratory, with respect to the studies of steroid excretion profiles in both breast and endometrial cancer patients, by using gas-liquid chromatographic analysis. These data demonstrate the importance of minor estrogens, including catechol estrogens, and of their ratios with the classical ones, in studies of steroid metabolism in both breast and endometrial cancer. New data concerning postmenopausal endometrial cancer are consistent with our previous observations and demonstrate the necessity of measuring these steroids directly in tumors and examining patterns of metabolism in vitro. In order to analyze steroid metabolic patterns in vitro, however, high-performance liquid chromatography rather than gas-liquid chromatography methods are preferable on account of their selectivity, specificity, sensitivity and capacity to handle labile materials. With the aim of providing methods suitable for the complete resolution and analysis of these complex natural mixtures a method of computer-aided optimization of HPLC has been developed and its practical utility has been tested.
本文简要回顾了迄今为止我们实验室通过气液色谱分析，在乳腺癌和子宫内膜癌患者类固醇排泄谱研究方面所取得的成果。这些数据表明，在乳腺癌和子宫内膜癌的类固醇代谢研究中，包括儿茶酚雌激素在内的微量雌激素及其与经典雌激素的比例具有重要意义。关于绝经后子宫内膜癌的新数据与我们之前的观察结果一致，表明直接在肿瘤中测量这些类固醇并在体外检查代谢模式的必要性。然而，为了在体外分析类固醇代谢模式，考虑到高效液相色谱法的选择性、特异性、灵敏度以及处理不稳定物质的能力，它比气液色谱法更可取。为了提供适用于完全分离和分析这些复杂天然混合物的方法，已经开发了一种高效液相色谱计算机辅助优化方法，并测试了其实际效用。
