Khan Abdul Saboor, Haider Muhammad Shafiq, Hassan Mubbashar, Husnain Ali, Yousuf Muhammad Rizwan, Ahmad Nasim
Department of Theriogenology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
Department of Theriogenology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
Anim Reprod Sci. 2018 Sep;196:28-34. doi: 10.1016/j.anireprosci.2018.06.012. Epub 2018 Jun 25.
The objective of this study was to evaluate the effect of equine chorionic gonadotropin (eCG) on the estrous response, ovulatory follicle development, ovulation rate, corpus luteum (CL) diameter, concentrations of progesterone (P4) and pregnancy per AI (P/AI) in CIDR-EB treated lactating anovular Nili-Ravi buffalo during the breeding season. Buffalo (n = 87), 60 to 200 Days postpartum were treated with CIDR at a random stage of the estrous cycle (Day 0) and assigned to two groups; with eCG (+eCG; n = 44), or without eCG (-eCG; n = 43). In both groups, each animal received 2 and 1 mg estradiol benzoate (EB) on Day 0 and then on Day 8, respectively. On Day 6, eCG and PGF were administered. On Day 7, CIDR were removed and FTAI was performed at 48 and 60 h later and pregnancy was diagnosed 35 Days post AI. The results revealed that eCG treatment increased (P < 0.05) the CL diameter on 15, 18, and 21 after the ovulation that resulted from imposing the estrous synchronization protocol than when eCG was not administered. Similarly, treatment with eCG resulted in an increased (P < 0.05) mean concentration of P4 as compared with animals not treated with eCG on Days 18 and 21 after the ovulation that occurred subsequent to imposing the estrous synchronization protocol. The ovulation rate was greater in eCG-treated buffalo as compared with those not treated with eCG (93% compared with 74%, respectively; P < 0.05). The P/AI was greater in buffalo treated with eCG than those not treated with eCG (56% compared with 31%, respectively; P < 0.05). In conclusion, the addition of eCG along with EB and P4 based treatments enhanced the estrous response, follicular and luteal function, ovulation rate, concentrations of P4, and P/AI in lactating anovular Nili-Ravi buffalo.
本研究的目的是评估马绒毛膜促性腺激素（eCG）对在繁殖季节使用CIDR-EB处理的哺乳期无排卵努里-拉维水牛的发情反应、排卵卵泡发育、排卵率、黄体（CL）直径、孕酮（P4）浓度及人工授精后的妊娠率（P/AI）的影响。87头产后60至200天的水牛在发情周期的随机阶段（第0天）用CIDR处理，并分为两组；一组使用eCG（+eCG；n = 44），另一组不使用eCG（-eCG；n = 43）。两组中，每头动物在第0天和第8天分别接受2毫克和1毫克苯甲酸雌二醇（EB）。在第6天，给予eCG和前列腺素F2α（PGF）。在第7天，取出CIDR，并在48小时和60小时后进行定时人工授精（FTAI），并在人工授精后35天诊断妊娠。结果显示，与未使用eCG时相比，在实施发情同步方案诱导排卵后，使用eCG处理使排卵后第15、18和21天的CL直径增加（P < 0.05）。同样，与未使用eCG处理的动物相比，在实施发情同步方案后排卵后的第18天和第21天，使用eCG处理使P4的平均浓度增加（P < 0.05）。与未使用eCG处理的水牛相比，使用eCG处理的水牛排卵率更高（分别为93%和74%；P < 0.05）。使用eCG处理的水牛的P/AI高于未使用eCG处理的水牛（分别为56%和31%；P < 0.05）。总之，在基于EB和P4的处理中添加eCG可增强哺乳期无排卵努里-拉维水牛的发情反应、卵泡和黄体功能、排卵率、P4浓度及P/AI。
