基于P4/E2/eCG的定时人工授精管理时季节对奶水牛繁殖性能的影响
Effect of season on dairy buffalo reproductive performance when using P4/E2/eCG-based fixed-time artificial insemination management.
作者信息
Monteiro Bruno Moura, Souza Diego Cavalcante de, Vasconcellos Guilherme de Souza Floriano Machado de, Carvalho Nelcio Antonio Tonizza de, Baruselli Pietro Sampaio
机构信息
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, SP, Brazil.
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, SP, Brazil; Rural Development Office, Registro, SP, Brazil.
出版信息
Theriogenology. 2018 Oct 1;119:275-281. doi: 10.1016/j.theriogenology.2018.07.004. Epub 2018 Jul 29.
This study aimed to compare the reproductive efficiency of dairy buffaloes subjected to TAI protocols based on progesterone, estrogen, and equine chorionic gonadotrophin (P4/E2+eCG) during the fall/winter (n = 168) and spring/summer (n = 183). Buffaloes received an intravaginal P4 device (1.0 g) plus estradiol benzoate (EB; 2.0 mg im) at a random stage of the estrous cycle (D-12). Nine days later (D-3), the P4 device was removed and buffaloes were given PGF (0.53 mg im sodium cloprostenol) plus eCG (400 IU im). GnRH (10 μg im buserelin acetate) was administered 48 h after P4 device removal (D-1). All animals were subjected to TAI 16 h after GnRH administration (D0). Frozen-thawed semen from one bull was used for all TAI, which were all performed by the same technician. Ultrasound examinations were performed on D-12 and D-3 to ascertain cyclicity (presence of CL), D-3 and D0 to measure the diameter of the dominant follicle (ØDF), D+10 to verify the ovulation rate and diameter of the corpus luteum (ØCL), and D+30 and D+45 to detect pregnancy rate (P/AI 30d and 45d, respectively) and embryonic mortality (EM). Fetal mortality (FM) was established between 45 days and birth, and pregnancy loss between 30 days and birth. There were significant differences between fall/winter and spring/summer only for cyclicity rate [76.2% (128/168) vs. 42.6% (78/183); P = 0.02]. The others variables did not differ between the seasons: ØDF on D-3 (9.6 ± 0.2 mm vs. 9.8 ± 0.2 mm; P = 0.35); ØDF on D0 (13.1 ± 0.2 mm vs. 13.2 ± 0.2 mm; P = 0.47); ovulation rate [86.9% (146/168) vs. 82.9% (152/182); P = 0.19]; ØCL on D+10 (19.0 ± 0.3 mm vs. 18.4 ± 0.3 mm, P = 0.20); P/AI on D+30 [66.7% (112/168) vs. 62.7% (111/177); P = 0.31]; P/AI on D+45 [64.8%% (107/165) vs. 60.2% (106/176); P = 0.37]; EM [1.8% (2/111) vs. 3.6% (4/110); P = 0.95]; FM [21.9% (18/82) vs. 8.0% (7/87); P = 0.13]; and PL [23.8% (20/84) vs. 12.1% (11/91); P = 0.13]. In conclusion, dairy buffaloes present similar reproductive efficiency in fall/winter and spring/summer when subjected to P4/E2/eCG-based protocol for TAI.
本研究旨在比较在秋冬季节（n = 168）和春夏季节（n = 183），采用基于孕酮、雌激素和马绒毛膜促性腺激素（P4/E2+eCG）的定时人工授精（TAI）方案的奶水牛的繁殖效率。水牛在发情周期的随机阶段（D-12）接受阴道内P4装置（1.0 g）加苯甲酸雌二醇（EB；2.0 mg肌肉注射）。九天后（D-3），取出P4装置，给水牛注射前列腺素F2α（0.53 mg肌肉注射氯前列醇钠）加eCG（400 IU肌肉注射）。在取出P4装置后48小时（D-1）注射促性腺激素释放激素（GnRH，10 μg肌肉注射醋酸布舍瑞林）。所有动物在注射GnRH后16小时（D0）进行定时人工授精。所有定时人工授精均使用来自同一头公牛的冻融精液，且均由同一名技术人员操作。在D-12和D-3进行超声检查以确定周期性（黄体的存在），在D-3和D0测量优势卵泡直径（ØDF），在D+10核实排卵率和黄体直径（ØCL），在D+30和D+45检测妊娠率（分别为P/AI 30天和45天）和胚胎死亡率（EM）。在45天至出生期间确定胎儿死亡率（FM），在30天至出生期间确定妊娠丢失率。仅在周期性率方面，秋冬和春夏之间存在显著差异[76.2%（128/168）对42.6%（78/183）；P = 0.02]。其他变量在不同季节之间没有差异：D-3时的ØDF（9.6±0.2 mm对9.8±0.2 mm；P = 0.35）；D0时的ØDF（13.1±0.2 mm对13.2±0.2 mm；P = 0.47）；排卵率[86.9%（146/168）对82.9%（152/182）；P = 0.19]；D+10时的ØCL（19.0±0.3 mm对18.4±0.3 mm，P = 0.20）；D+30时的P/AI[66.7%（112/168）对62.7%（111/177）；P = 0.31]；D+45时的P/AI[64.8%（107/165）对60.2%（106/176）；P = 0.37]；EM[1.8%（2/111）对3.6%（4/110）；P = 0.95]；FM[21.9%（18/82）对8.0%（7/87）；P = 0.13]；以及PL[23.8%（2'/84）对12.1%（11/91）；P = 0.13]。总之，当采用基于P4/E2/eCG的定时人工授精方案时，秋冬和春夏季节的奶水牛具有相似的繁殖效率。
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