Dichter M A, Weiner H L, Fields B N, Mitchell G, Noseworthy J, Gaulton G, Greene M
Ann Neurol. 1986 Jun;19(6):555-8. doi: 10.1002/ana.410190606.
A syngeneic monoclonal antiidiotype directed against the idiotype of an antireovirus type 3 hemagglutinin demonstrates several of the biological actions of the original viral hemagglutinin and binds to rat and murine cortical neurons grown in dissociated cell culture. Receptor-bearing neurons appear within 24 hours of plating in cultures from mouse or rat cortex taken on embryonic day 15; these neurons are demonstrable for the duration of the culture life span (4 to 8 weeks). When cortical cultures are incubated with antiidiotype before or during exposure to reovirus, the antiidiotype protects neurons from type 3 infection without inhibiting infection of nonneuronal cells with either type 3 or type 1. Thus an antibody directed against a viral receptor can prevent infection of receptor-bearing cells without directly neutralizing the virus.
一种针对抗3型呼肠孤病毒血凝素独特型的同基因单克隆抗独特型抗体，表现出原始病毒血凝素的多种生物学活性，并能与在解离细胞培养中生长的大鼠和小鼠皮质神经元结合。在取自胚胎第15天的小鼠或大鼠皮质培养物中接种后24小时内，即可出现带有受体的神经元；在培养寿命（4至8周）期间均可检测到这些神经元。当在暴露于呼肠孤病毒之前或期间用抗独特型抗体孵育皮质培养物时，抗独特型抗体可保护神经元免受3型感染，而不会抑制3型或1型病毒对非神经元细胞的感染。因此，针对病毒受体的抗体可以预防带有受体细胞的感染，而无需直接中和病毒。
