Noseworthy J H, Fields B N, Dichter M A, Sobotka C, Pizer E, Perry L L, Nepom J T, Greene M I
J Immunol. 1983 Nov;131(5):2533-8.
We have prepared and characterized a syngeneic monoclonal anti-idiotype directed against the idiotype (Id3) of the anti-reovirus type 3 hemagglutinin B cell hybridoma 9BG5. We have shown this monoclonal antibody to be anti-idiotypic in a series of inhibition studies with purified sigma 1 (hemagglutinin) protein and with absorption studies on the idiotypic R1.1 cell line. That the idiotype Id 3 defined by the monoclonal anti-idiotype is distinct from H-2 was demonstrated by its presence on an idiotype-expressing H-2 negative cell line (R1.E). Binding of the anti-idiotype is relatively tunicamycin resistant and pronase/trypsin sensitive, which suggests that the recognition site of the anti-idiotypic receptor is poorly glycosylated. The presence of idiotype-like receptors on reovirus type 3 susceptible neurons may have important implications for the presence of related gene families between neural and lymphoid tissue. The implications of recognition by neuronal cells of an anti-idiotypic monoclonal antibody directed against the reovirus 3 attachment receptor for the pathogenesis of viral-induced auto-immunity are discussed.
我们制备并鉴定了一种同基因单克隆抗独特型抗体，它针对抗呼肠孤病毒3型血凝素B细胞杂交瘤9BG5的独特型（Id3）。在一系列用纯化的σ1（血凝素）蛋白进行的抑制研究以及对独特型R1.1细胞系的吸收研究中，我们已证明这种单克隆抗体具有抗独特型特性。通过在表达独特型的H-2阴性细胞系（R1.E）上存在该独特型，证明了由单克隆抗独特型所定义的独特型Id 3与H-2不同。抗独特型抗体的结合相对耐衣霉素且对链霉蛋白酶/胰蛋白酶敏感，这表明抗独特型受体的识别位点糖基化程度较低。呼肠孤病毒3型易感神经元上存在独特型样受体可能对神经组织和淋巴组织之间相关基因家族的存在具有重要意义。本文讨论了神经元细胞识别针对呼肠孤病毒3型附着受体的抗独特型单克隆抗体对病毒诱导的自身免疫发病机制的影响。
