Sundaresan G, Abraham Robinson J J, Appa Rao V, Narendra Babu R, Govind V, Meti Mahantesh F
Department of Livestock Products Technology (Meat Science), Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007 India.
J Food Sci Technol. 2018 Sep;55(9):3439-3445. doi: 10.1007/s13197-018-3253-4. Epub 2018 Jul 18.
A study was conducted for extraction of chondroitin sulphate (CS) from buffalo tracheal, nasal and joint cartilages. CS was extracted from cartilages using 0.25% papain digestion, dialyzed, precipitated with 10% TCA and finally lyophilized to dry powder. Dimethylmethylene blue assay was performed to estimate the quantity of CS extracted. Identification of extracted CS was performed with SDS-PAGE and Fourier transforms infrared spectroscopy (FTIR). SDS-PAGE analysis of extracted CS revealed similar electrophoretic pattern to that of standard and the molecular weight ranged from 5 to 20 kDa. FTIR spectra of extracted CS revealed presence of characteristic peaks of -CONH vibration of amide group, coupling of C-O stretching vibration, S=O stretching vibrations and -C-O-S molecules confirms the CS moiety. It can be concluded that extraction method adopted could efficiently be utilized for the extraction of CS from buffalo by-products like tracheal, nasal and joint cartilages.
开展了一项从水牛气管、鼻软骨和关节软骨中提取硫酸软骨素（CS）的研究。使用0.25%木瓜蛋白酶消化法从软骨中提取CS，进行透析，用10%三氯乙酸沉淀，最后冻干成干粉。采用二甲基亚甲蓝法测定提取的CS的量。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和傅里叶变换红外光谱（FTIR）对提取的CS进行鉴定。提取的CS的SDS-PAGE分析显示其电泳图谱与标准品相似，分子量范围为5至20 kDa。提取的CS的FTIR光谱显示存在酰胺基团的-CONH振动、C-O伸缩振动的耦合、S=O伸缩振动以及-C-O-S分子的特征峰，证实了CS部分。可以得出结论，所采用的提取方法可有效地用于从水牛气管、鼻软骨和关节软骨等副产品中提取CS。
