Moghaddas Kia Ehsan, Alizadeh Mohammad, Esmaiili Mohsen
1Food Science and Technology Department, Maragheh University of Medical Sciences, Maragheh, Iran.
2Food Science and Technology Department, Urmia University, Urmia, Iran.
J Food Sci Technol. 2018 Sep;55(9):3657-3664. doi: 10.1007/s13197-018-3294-8. Epub 2018 Aug 2.
In this study probiotic microcapsules with three different shell compositions were produced through enzymatic gelation of skim milk powder by rennet, skim milk powder by transglutaminase and sodium caseinate by transglutaminase. Fabricated microcapsules and free cells were incorporated into Iranian UF Feta cheese with different salt levels. Viability of (LAFTI ), antioxidative capacity, ripening index, titrable acidity, salt content and texture profile analysis (TPA test) parameters including hardness, cohesiveness and stringiness were monitored during 45 days of storage time. Rennet based encapsulation was the most efficient method and could keep viable in all cheese samples (> 7 log CFU/g) at the end of storage time. Proteolysis pattern and acidification rate were strongly influenced by shell composition, salt level and storage time. Hardness and stringiness of probiotic cheese samples were influenced by shell composition of microcapsules and storage time but cohesiveness was only dependent on storage time. Therefore, storage time was the only effective factor on free radical scavenging activity of cheese samples.
在本研究中,通过用凝乳酶对脱脂奶粉进行酶凝胶化、用转谷氨酰胺酶对脱脂奶粉进行酶凝胶化以及用转谷氨酰胺酶对酪蛋白酸钠进行酶凝胶化,制备了三种不同壳组成的益生菌微胶囊。将制备的微胶囊和游离细胞添加到不同盐含量的伊朗超滤费塔奶酪中。在45天的储存期内,监测了嗜酸乳杆菌(LAFTI)的存活率、抗氧化能力、成熟指数、可滴定酸度、盐含量以及质地剖面分析(TPA测试)参数,包括硬度、内聚性和黏性。基于凝乳酶的包封是最有效的方法,在储存期结束时,所有奶酪样品中的嗜酸乳杆菌(LAFTI)存活率均能保持在>7 log CFU/g。蛋白水解模式和酸化速率受壳组成、盐含量和储存时间的强烈影响。益生菌奶酪样品的硬度和黏性受微胶囊壳组成和储存时间的影响,但内聚性仅取决于储存时间。因此,储存时间是影响奶酪样品自由基清除活性的唯一有效因素。