Chen HM, Muramoto K, Yamauchi F, Fujimoto K, Nokihara K
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai 981, Japan, and Shimadzu Scientific Research Inc., Tokyo 101, Japan.
J Agric Food Chem. 1998 Jan 19;46(1):49-53. doi: 10.1021/jf970649w.
The properties of 22 synthetic peptides containing histidine, which were designed on the basis of the antioxidative peptide (Leu-Leu-Pro-His-His) derived from proteolytic digests of a soybean protein, were examined with regard to their antioxidative activity against the peroxidation of linoleic acid and the scavenging effects on active oxygen and free radical species. The antioxidative activities of these peptides in an emulsion oxidation system using 2,2'-azobis(2-amidinopropane) dihydrochloride as a radical initiator correlated well within an aqueous system. Although the histidine-containing peptides had a quenching activity on singlet oxygen, they did not show antioxidative activity in an 2,2'-azobis(2,4-dimethylvaleronitrile)-induced oxidation system or scavenging effects on 1,1-diphenyl-2-picrylhydrazyl radical and superoxide. The metal-ion chelating activities and the hydrophobicities of these peptides showed no direct correlation with their antioxidative activities. Leu-Leu-Pro-His-His was modified with a hydroxyl radical in an aqueous ethanol system during the peroxidation of linoleic acid.
对基于大豆蛋白蛋白水解物衍生的抗氧化肽（Leu-Leu-Pro-His-His）设计的22种含组氨酸的合成肽的性质进行了研究，考察了它们对亚油酸过氧化的抗氧化活性以及对活性氧和自由基的清除作用。在使用2,2'-偶氮二异丁脒盐酸盐作为自由基引发剂的乳液氧化体系中，这些肽的抗氧化活性在水体系中具有良好的相关性。尽管含组氨酸的肽对单线态氧有猝灭活性，但它们在2,2'-偶氮二（2,4-二甲基戊腈）诱导的氧化体系中未表现出抗氧化活性，对1,1-二苯基-2-苦基肼自由基和超氧阴离子也没有清除作用。这些肽的金属离子螯合活性和疏水性与其抗氧化活性没有直接相关性。在亚油酸过氧化过程中，Leu-Leu-Pro-His-His在乙醇水溶液体系中被羟基自由基修饰。
