Fernandez María Verónica, Jagus Rosa Juana, Agüero María Victoria
1Consejo Nacional de Investigaciones Científica y Técnicas (CONICET), Instituto de Tecnologías y Ciencias de la Ingeniería (INTECIN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina.
2Laboratorio de Microbiología Industrial: Tecnología de alimentos, Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Ciudad Universitaria, Intendente Güiraldes 2620 (C1428EGA), Buenos Aires, Argentina.
J Food Sci Technol. 2018 Sep;55(9):3665-3674. doi: 10.1007/s13197-018-3295-7. Epub 2018 Jul 12.
Nisin (Ni), natamycin (Na), green tea extract (GTE) and their combinations were evaluated for controlling beet leaves' native microbiota as well as and external contaminations. Antimicrobial effectiveness was evaluated through in vitro and in vivo studies. In the in vitro studies, GTE treatment (0.85%) completely eliminated growth of native microbiota, reduced from values of 8.5-3.5 log from 24 h onwards and reduced below detection limit (DL) after 72 h. Ni (500 IU/mL) was the most effective against (7 log CFU/mL reduction) and its combination with GTE presented significant interactions for mesophilic aerobic bacteria (MAB) and control. Na (200 ppm) alone or in combination with GTE did not show antimicrobial activity against microorganisms under study. Additionally in vivo evaluation showed that 2.5-5% GTE concentrations are needed to achieve significant inhibitory effects on MAB, and . Furthermore, the best results for MAB and control were obtained with the GTE5 + Ni treatment. This study revealed that GTE, either alone or combined with nisin, is a highly promising option with potential for reducing or preventing the growth of pathogenic and spoilage microorganisms present in leafy vegetables, specifically in beet leaves.
评估了乳酸链球菌素（Ni）、纳他霉素（Na）、绿茶提取物（GTE）及其组合对甜菜叶原生微生物群以及外部污染的控制效果。通过体外和体内研究评估抗菌效果。在体外研究中，GTE处理（0.85%）完全消除了原生微生物群的生长，从24小时起将其数量从8.5 - 3.5 log减少，并在72小时后将其减少到检测限（DL）以下。Ni（500 IU/mL）对[具体微生物名称未明确]最有效（减少7 log CFU/mL），并且其与GTE的组合对嗜温需氧菌（MAB）和[具体微生物名称未明确]对照呈现出显著的相互作用。单独的Na（200 ppm）或与GTE组合对所研究的微生物均未显示出抗菌活性。此外，体内评估表明，需要2.5 - 5%的GTE浓度才能对MAB、[具体微生物名称未明确]和[具体微生物名称未明确]产生显著的抑制作用。此外，GTE5 + Ni处理对MAB和[具体微生物名称未明确]对照取得了最佳结果。这项研究表明，GTE单独使用或与乳酸链球菌素联合使用，是一种非常有前景的选择，具有减少或预防叶菜类蔬菜，特别是甜菜叶中存在的致病和腐败微生物生长的潜力。
