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丛枝菌根真菌改善镉污染土壤中番茄植株的生长、光合作用并保护其叶片的光系统II

Arbuscular Mycorrhizal Fungi () Improves Growth, Photosynthesis and Protects Photosystem II in Leaves of L. in Cadmium Contaminated Soil.

作者信息

Zhang Huihui, Xu Nan, Li Xin, Long Jinghong, Sui Xin, Wu Yining, Li Jinbo, Wang Jifeng, Zhong Haixiu, Sun Guang Y

机构信息

School of Resources and Environmental Science, Northeast Agricultural University, Harbin, China.

Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences, Harbin, China.

出版信息

Front Plant Sci. 2018 Aug 13;9:1156. doi: 10.3389/fpls.2018.01156. eCollection 2018.

Abstract

In this study, the effects of inoculating arbuscular mycorrhizal fungi () on the growth, chlorophyll content, photosynthetic gas exchange parameters, and chlorophyll fluorescence characteristics of L. in cadmium (Cd) contaminated soil were investigated. The results showed that the root vigor of declined, while the chlorophyll content significantly decreased with the increase of Cd content, especially the chlorophyll a content in leaves. The photosynthetic carbon assimilation capacity and PSII activity of leaves were also significantly inhibited by Cd stress, especially the electron transfer at the receptor side of PSII, which was more sensitive to Cd stress. The infection level of on roots was relatively high and inoculation with increased the mycorrhizal infection rate of roots up to 50-70%. Due to the impact of the mycorrhizal infection, the Cd content in roots was significantly increased compared to non-inoculated treatment; however, the Cd content in the aboveground part of was not significantly different compared to the non-inoculated treatment. After inoculation with , the root vigor of increased to some extent, alleviating the chlorophyll degradation in leaves under Cd contaminated soil. Infection with can improve the stoma limitation of leaves in Cd contaminated soil and increase the non-stomatal factors including the tolerance of its photosynthetic apparatus to Cd, to improve photosynthetic capacity. infection can improve the photosynthetic electron transport capacity of PSII in leaves under Cd stress and promotes the activity of the oxygen-evolving complex to different degrees at the donor side of PSII and the electron transport capacity from Q to Q on the receptor side of PSII. Thus, this guarantees that leaves inoculated with in Cd contaminated soil have relatively higher PSII activity. Therefore, inoculation with can improve the capacity of Cd tolerance of with regard to various aspects, such as morphological characteristics and photosynthetic functions, and reduce the toxicity of Cd on .

摘要

本研究调查了接种丛枝菌根真菌对镉(Cd)污染土壤中黑麦草生长、叶绿素含量、光合气体交换参数及叶绿素荧光特性的影响。结果表明,随着Cd含量增加,黑麦草根系活力下降,叶绿素含量显著降低,尤其是叶片中的叶绿素a含量。Cd胁迫还显著抑制了黑麦草叶片的光合碳同化能力和PSII活性,特别是PSII受体侧的电子传递,其对Cd胁迫更为敏感。丛枝菌根真菌对黑麦草根系的侵染水平相对较高,接种后黑麦草根系的菌根侵染率提高至50 - 70%。由于菌根侵染的影响,与未接种处理相比,接种后黑麦草根系中的Cd含量显著增加;然而,地上部分的Cd含量与未接种处理相比无显著差异。接种丛枝菌根真菌后,黑麦草根系活力有一定程度提高,缓解了Cd污染土壤中黑麦草叶片叶绿素的降解。接种丛枝菌根真菌可改善Cd污染土壤中黑麦草叶片的气孔限制,增加包括光合机构对Cd耐受性在内的非气孔因素,从而提高光合能力。接种丛枝菌根真菌可提高Cd胁迫下黑麦草叶片PSII的光合电子传递能力,并在PSII供体侧不同程度地促进放氧复合体的活性以及PSII受体侧从Q到Q的电子传递能力。因此,这保证了在Cd污染土壤中接种丛枝菌根真菌的黑麦草叶片具有相对较高的PSII活性。所以,接种丛枝菌根真菌可在形态特征和光合功能等多个方面提高黑麦草对Cd的耐受能力,降低Cd对黑麦草的毒害作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb8f/6099091/4b179919ac5b/fpls-09-01156-g001.jpg

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