[Synthesis and cloning of a DNA fragment containing a probable site for eukaryotic mRNA binding to ribosome].

A series of oligonucleotides, including two polynucleotides of 33 bases long, were synthesized by a solid-phase phosphotriester method. Potassium salt of 3-nitro-1,2,4-triazole in the presence of 18-crown-6 ether was used as nucleophilic catalyst. The partly complementary polynucleotides were elongated by DNA-polymerase I (Klenow fragment) to the full duplex, which was digested with SalGI and was inserted into a plasmid pUR222. Phe synthesized DNA fragment precedes the gene of human gamma-interferon in the chromosome and contains the site for mRNA binding to ribosome.