人胰岛素基因的合成。第四部分。用于DNA克隆和序列分析的新型合成脱氧核糖寡核苷酸接头和引物。
Synthesis of the human insulin gene. Part IV. New synthetic deoxyribooligonucleotide adaptors and primer for DNA cloning and sequence analysis.
作者信息
Narang S A, Brousseau R, Hsiung H M, Sung W, Scarpulla R, Ghangas G, Lau L, Hess B, Wu R
出版信息
Nucleic Acids Symp Ser. 1980(7):377-85.
The chemical synthesis of four new deoxyribooligonucleotides to be used as adaptors in molecular cloning of DNA for expression studies is described. These are (i) start-adaptor, (ii) stop-adaptor, (iii) conversion adaptor to insert ribosomal binding site, and (iv) retrieving adaptor. We have also synthesized a 19-bases-long primer, 5'-TTGTAAAACGACGGCCAGT-3' to increase the speed of DNA sequence analysis and also demonstrated its application using single-stranded M13 DNa vector.
本文描述了四种新的脱氧核糖寡核苷酸的化学合成方法，这些寡核苷酸将用作DNA分子克隆中的衔接子，用于表达研究。它们分别是：（i）起始衔接子；（ii）终止衔接子；（iii）用于插入核糖体结合位点的转换衔接子；（iv）回收衔接子。我们还合成了一个19个碱基长的引物，5'-TTGTAAAACGACGGCCAGT-3'，以提高DNA序列分析的速度，并使用单链M13 DNA载体展示了其应用。
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