Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, Nebraska.
Biomedical Engineering Program, University of Nebraska, Lincoln, Nebraska.
J Biomed Mater Res A. 2018 Dec;106(12):3135-3142. doi: 10.1002/jbm.a.36508. Epub 2018 Aug 27.
Mesenchymal stem cells (MSCs) have been widely studied for tissue engineering and treating diseases in laboratories, clinical trials, and clinics. Fibrin matrices are often used to culture MSCs or increase the retention of MSCs at the injection site. However, fibrins made with the human plasma derived fibrinogen have high cost and risk of human pathogen transmission. In this article, we studied if fibrin matrices made with recombinant human fibrinogen, recombinant human thrombin, and recombinant human factor XIII could be used to culture and deliver MSCs. We systematically investigated the relationships between the fibrin matrix formulation, its nanostructure, and the behaviors of the cells in the matrix including the cell morphology, viability, and growth. We found that the fibrinogen concentration significantly affected the matrix structure and cell behaviors. We then used an optimized fibrin matrix to deliver human MSCs into mice subcutaneously. We found that the matrix could significantly enhance the retention of MSCs at the injection site. To our best knowledge, this is the first study on using fibrin matrices made with entirely recombinant proteins for culturing and delivering MSCs. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3135-3142, 2018.
间充质干细胞（MSCs）已在实验室、临床试验和临床中广泛研究用于组织工程和治疗疾病。纤维蛋白基质常用于培养 MSCs 或增加 MSCs 在注射部位的保留。然而，用人血浆来源的纤维蛋白原制成的纤维蛋白具有高成本和人类病原体传播的风险。在本文中，我们研究了是否可以使用重组人纤维蛋白原、重组人凝血酶和重组人因子 XIII 制成的纤维蛋白基质来培养和输送 MSCs。我们系统地研究了纤维蛋白基质配方、其纳米结构以及基质中细胞行为之间的关系，包括细胞形态、活力和生长。我们发现纤维蛋白原浓度显著影响基质结构和细胞行为。然后，我们使用优化的纤维蛋白基质将人 MSCs 递送至小鼠皮下。我们发现该基质可显著增强 MSCs 在注射部位的保留。据我们所知，这是第一项使用完全重组蛋白制备的纤维蛋白基质培养和输送 MSCs 的研究。© 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3135-3142, 2018.
